Hi all !! I am working with two data sets of same protein (130a/a) with resolutions 2.8 and 3.2 A. In both the cases the density for 100-130 aa is not very clear.. it forms couple of helices.. i can see a long tube going but it is feature less.. It is a MR solution.. i have tried TLS refinement with different TLS groups.. but no luck... i have also tried DM but not much improvement... Data looks clean without any twinning... R and Rfee are around 22.8 and 28.5 for both the structures... Any suggestions??
regards John
