Hi Heidi,
why don't you just install the latest binary distribution? That's much
less hassle ... just go to
http://www.ysbl.york.ac.uk/~emsley/software/binaries/nightlies/pre-release/
and grab e.g.
coot-0.5-pre-1-revision-1003-binary-Linux-i386-fedora-5.tar.gz
(Fedora 6 corresponds to RHEL5
And how will you know if they are the calcium citrate xtals Sam asked about,
and not
sodium citrate xtals? Sodium citrate in the Hampton Crystal Screen condition
will
crystallize out at 4 degrees. It's solubiility is pH and temperature dependent.
You'll need to go to your friendly neighborhood
Jim Pflugrath wrote:
> That's an easy hypothesis to test. Simply set up your drops with the
> same conditions except without protein and see if you get crystals.
Not always. I've had a few cases where the protein turns out to be the
essential additive in order to obtain salt crystals!
Looking
That's an easy hypothesis to test. Simply set up your drops with the same
conditions except without protein and see if you get crystals. Please let
us know the results. Thanks!
Jim
On Tue, 29 Apr 2008, Sam Stephenson wrote:
Are calcium citrate crystals a common false positive in trays with
Are calcium citrate crystals a common false positive in trays with up to 200mM
of each? There is absolutely no phosphate in the trays so I'm almost positive
they're not calcium phosphate.
Cheers,
Sam
-
Be a better friend, newshound, and know-it-all with Y
You can integrate them as separate wedges (in different directories),
then later merge them (XDS_ASCII.HKL) in Xscale. Make sure though you
have the orientation right and starting angles etc.
You'll have to first refine one wedge, then once you are happy with it
copy the GXPARM.XDS into the othe
Hi all,
is it possible to input discontinuous data wedges into XDS (obtained from for
example inverse beam sweeps)? (So wedge se1 goes from 0-90 deg (image 1-90),
se2 from 180-270 (image 1-90), etc). Or do I have to rename everything so that
I get one data file in which the rotation ranges are
Dear All,
I have a protein structure I am trying to refine using Refmac. There are
~158,000 atoms modeled (no waters) in the asymetric unit with ~495,000
reflections. There is data to 3.0A resolution with the 2 sigma cutoff at 3.5A.
I have four NCS copies in my asymetric unit and when I speci
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Institute of Molecular Biology and Departments of Physics
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University of Oregon
A postdoctoral position is immediately
available for structural
studies of the CagA protein from Helicobacter pylori, which causes
oncogenic
transformations in
Regarding reason 1, you can configure sshd to block root from logging in
remotely ("PermitRootLogin no" in /etc/ssh/sshd_config). I was surprised to
see that this wasn't the default in some systems, but made sure to turn it on
after I saw my first dictionary attack (and since).
Not that I disa
Hi all,
I am looking for a software that can help me draw a helical wheel
diagram with appropriate coloring of the residues for a few peptides.
Does anyone knows a cool program for the same.
thanks
Neeraj
--
Neeraj Kapoor
TPCB Graduate Fellow
Sakmar Lab/ Molecular Biology & Biochemistry
Sickmier, Allen wrote:
Hi All,
Is there a way to draw surfaces in coot, or is there a nice associated
program that will draw surfaces in coot.
I know about the dotted surface but I was looking for more of a
continuous surface. Like insight or pymol.
Hi Allen,
Yes there is a way (if
Hi All,
Is there a way to draw surfaces in coot, or is there a nice associated program
that will draw surfaces in coot.
I know about the dotted surface but I was looking for more of a continuous
surface. Like insight or pymol.
Allen
A postdoctoral position is available in the group of Roger Williams
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and reconstitute this
I usually install crystallographic software as administrator using
sudo under /usr/local/xtal and make it readable/executable for all
users. This is sufficient in my experience.
Best regards,
Dirk.
Am 29.04.2008 um 12:51 schrieb PhilEvans:
I've not found it necessary to activate root. I've
I've not found it necessary to activate root. I've installed quite a
lot of stuff in /usr/local but found it useful to make /usr/local
belong to me (sudo chown -R /usr/local). With fink, I use sudo &
not found it inconvenient (you can use "sudo -s" to go into admin mode
until further notic
On Apr 29, 2008, at 2:25 AM, Andreas Förster wrote:
Why working with sudo is safer than working as root is not clear to
me. After all, the danger is not in root but in the uneducated user.
The danger *is* in root, if for no other reason than you must type
"sudo" before you issue that comman
On Apr 29, 2008, at 11:25, Andreas Förster wrote:
Why working with sudo is safer than working as root is not clear to
me. After all, the danger is not in root but in the uneducated
user. If you're paranoid, you can keep using sudo until you get
stuck and then switch to root.
There are t
Without having followed this thread, wouldn't the whole procedure you
describe also work with
1. Open a terminal
2. type 'sudo passwd root'
3. Enter the current users password (to allow for the sudo-command)
4. Enter the new root password (twice)
This should work under all versions of MacOSX.
A few month's back, and a few moments before almost throwing my
Powerbook out the window, I enabled the root account, as officially
described (http://docs.info.apple.com/article.html?artnum=106290):
How to enable the root user
Mac OS X 10.5 or later
1. From the Finder's Go menu, choose Uti
Dear Jorge,
I was unable to reproduce the error you report with 7.0.1.
I selected an MTZ file, then chose 'use' FreeR flag and I run the job.
I get:
#CCP4I VERSION CCP4Interface 1.4.4.2
#CCP4I SCRIPT LOG arp_solvent
#CCP4I DATE 29 Apr 2008 10:56:28
#CCP4I USER Tassos
...
mtz labels taken: FP
REMINDER:
A workshop entitled "HTP Crystallization and information Management"
will be held on 18-20 June 2008 at NKI ,Amsterdam. This jointly sponsored workshop TEACH-SG and BIOXHIT is open to all applicants. The course will include lectures and practical session on HTP nano-crystallization, opt
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