Without knowing the resolution, you could try to use TLS refinement (if you
haven't done it
already) and see if the maps improve and allow you to build more/better. If
the resolution is
reasonable and you can not lower the R & Rfree you should check whether
twinning is
possible. It is common
Dear Jeff:
The sequence here is NYT. I think it is glycosylated here, isn't it?
Thanks a lot.
On Jan 8, 2008 11:37 AM, Jeff Lee <[EMAIL PROTECTED]> wrote:
> Hi,
> Do you know what is the protein sequence in this region. If it is
> glycosylated, it should have a distinctive motif (N-X-S/T). You
You should check if this residue is a part of a glycosylation pattern
(either via crude computational sequence analysis, or if you're lucky - by
digest and peptide MS).
The density *looks like* a disordered sugar but at 2.6A this is a very tough
call.
Artem
_
From: CCP4 bulletin b
Depending on your data quality and the nature of the crystal it might be
that you cannot reduce R/Rfree any further.
Low R/Rfree is not the goal of refinement and model building - at least
not for the humans doing it. The goal is to get as good a model as
possible, and if your data are not very
The space group is P212121
There are four chains in one asymmetric unit: two A chains and two B chains.
-
Never miss a thing. Make Yahoo your homepage.
I have a large molecule to refine.There are about three thousand residues in
one asymmetric unit.
Except for the lack of electron densities in several places, where the
loops have been deleted, the rest parts fits well with density. But the R free
and R work are all beyond 0.30 (R free = 0.35,
Hi,
I'm looking for a program to improve a low homology sequence alignment using
the known (but unpublished) structure of one of the sequences. Programs like
Verify3D and CPROF (Eisenberg's lab) generate the environmental profile of a
structure, but then how do I apply that to other sequences t
Just a final reminder. The deadline for submitting abstracts (posters
and talks) has been extended to January 12 (this Saturday). Students
are strongly encouraged to submit abstracts and will be considered
for the Etter Student Lecturer Award if invited to speak. There is
still room in the
The Feb issue of AMS Notices (American Mathematical Society)
contains an article of possible interest to crystallographers.
http://www.ams.org/notices/200802/
"Crystals That Nature Might Miss Creating" - Toshikazu Sunada
I confess myself perplexed as to what is actually being
described in
On Mon, 2008-01-07 at 18:22 +, Erin Curry wrote:
> Tired of embarrassing incidents at the beamline?...
Alright, who's been telling stories?
-
===
With the single exception of Cornell, there is not a college in the
United
Dear all,
I am working on a multidomain structure in P31 with two
copies in the asymmetric unit and pseudo-P3121 symmetry - the NCS is a
twofold relating most of copy A to copy B but one domain violates the
higher symmetry. I have placed all the NCS-obeying domains of A and B,
plus co
Scientist Position in Protein Crystallography - Brazilian Synchrotron Light
Laboratory (LNLS)
LNLS is a 1.37 GeV synchrotron source located in Campinas, Brazil, at 90 km
NW from São Paulo. It is the only synchrotron laboratory in Latin America
and has two beam lines dedicated to Macromolecular
Hi Jessica:
Thanks for posting these gastroeneterology and dermatology adverts to the CCP4
bulletin board. Many of in macromolecular X-ray crystallography are
contemplating just such a career change, and although the retraining procedure
may take 7 to 10 years, I am sure you would be willing t
Post Doctoral Research Assistant in Ion Channel Biochemistry & Structural
Biology
Grade 7: £26,666 - £32,796 pa
An experienced post doctoral research assistant is required to work on
structural studies of an exciting new class of prokaryotic ion channels.
The successful applicant will join the P
2008 American Crystallographic Association Meeting
01.04 Structural Enzymology
Tuesday afternoon, June 3, 2008
Organized by Drs. Allen M. Orville and Carrie M. Wilmot
The abstract deadline for the 2008 ACA meeting in Knoxville, TN,
including our Structural Enzymology session, was extended t
Dear all,
Rigaku, the world's largest analytical X-ray company, is seeking an
application scientist to work with our ACTOR team. ACTOR, Automated
Crystal Transport, Orientation and Retrieval, is the most popular
automated sample changer for X-ray crystallography. You will help
install, mainta
To all those interested:
One other position available for a Dermapathologist in Delaware.
This position is for a BC or BE physician. Only candidates from Dermatology
(not Pathology) will be considered. Position is Monday thru Friday, daytime,
call rotates every 7th week. Residency on up. Will
To all those interested,
We have a 2 current openings in the same facility for a Gastroenterology
specialist in Tennessee. Consultation status with the health system and will
follow up with patients in the hospital after they are admitted.
?
Benefits include
NO call
Endoscopy procedure lab
This year, the ICCBM conference will take place in Cancun Mexico, May
6-9 (http://www.iccbm12.com.mx/) and a 3-day workshop (about 30-36
participants) will precede this conference on the crystallization of
biological macromolecules.
For information regarding workshop/school and conference, plea
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