[ccp4bb] ncs phased refinement

2007-12-07 Thread Raja Dey
Hi All, I was trying to run NCS Phased refinement in the gui of CCP4 (Refmac_5.2.0005) and getting the following error. I just pasted below the last part of the log file. Any help is greatly appreciated. Tarak ROTATION MATRIX -0.5

Re: [ccp4bb] source for forceps/clamps that fit around cryovials

2007-12-07 Thread William Scott
On Fri, 7 Dec 2007 13:22:03 -0500 [EMAIL PROTECTED] wrote: CCP4 bulletin board wrote on 12/07/2007 12:43:55 PM: > could anyone drop the name of a source for those tweezer-like clamps whose > ends fit perfectly around a Nunc/CryoCap cryovial? one very famous so

Re: [ccp4bb] source for forceps/clamps that fit around cryovials

2007-12-07 Thread Matthew . Franklin
CCP4 bulletin board wrote on 12/07/2007 12:43:55 PM: > could anyone drop the name of a source for those tweezer-like clamps whose > ends fit perfectly around a Nunc/CryoCap cryovial? one very famous source > seems not to have them. > > these are not the locking clamps or thick metal tongs, but a

[ccp4bb] source for forceps/clamps that fit around cryovials

2007-12-07 Thread Bryan W. Lepore
could anyone drop the name of a source for those tweezer-like clamps whose ends fit perfectly around a Nunc/CryoCap cryovial? one very famous source seems not to have them. these are not the locking clamps or thick metal tongs, but are more like tweezers with the ends curved into half-circles

[ccp4bb] summary: heavy metal source update

2007-12-07 Thread Joyce, Gordon M.(NIH/NIAID) [F]
So got the triethyl lead acetate. Thanks again to everyone. Gordon triethyl lead acetate by it's trickier name Acetoxytriethyllead(IV): http://www.sigmaaldrich.com/catalog/search/ProductDetail/ALDRICH/116823

Re: [ccp4bb] SUMMARY: PEG MW vs. cryoprotectivity

2007-12-07 Thread Juergen Bosch
Something else I thought to include in my earlier email, not sure if you guys are aware of this nice database. Search for your main precipitant and see what others did to protect their poor crystals. http://idb.exst.jaxa.jp/db_data/protein/search-e.php Juergen -- Jürgen Bosch University of Wa

[ccp4bb] Purification using GST column at low pH

2007-12-07 Thread E rajakumar
Dear All Sorry for non-crystallography question. I am purifying a protein using GST column. The gel filtration chromatography is indicating that the protein is aggregating at pH more than 7.0. I am planning to purify the protein at the pH around 6.0. So, I want to know the affinity of GST-fusion pr

[ccp4bb] heavy atom source summary

2007-12-07 Thread Joyce, Gordon M.(NIH/NIAID) [F]
Thank you for the many useful replies which resulted in finding sources for these two compounds. methylmercury acetate at Pfaltz & Bauer Trimethyllead acetate at Chemos GmbH Still at a loose end for a commercial source for the Triethyllead acetate though. It used to be carried

[ccp4bb] deadline for proposals (12th Dec) for beamtime at BM14 - Feb/Mar 2008

2007-12-07 Thread Martin A. Walsh
Dear all, deadline for submission of proposals for beamtime at bm14 is fast approaching –with the deadline set for next Wednesday (12th December) Proposals arriving before 17:05 on this date will be considered for scheduling in the feb/Mar 2008 synchrotron run at the ESRF. Follow this link

[ccp4bb] Postdoctoral Research Position in Oxford

2007-12-07 Thread Erika Mancini
Postdoctoral Research Assistant position http://www.nature.com/naturejobs/science/jobs/36168 Applications are invited for an enthusiastic and motivated postdoctoral research scientist to join Dr. Erika Mancini’s group at the Division of Structural Biology, Wellcome Trust Centre for Human Geneti

[ccp4bb] Refmac extra restraint

2007-12-07 Thread Kristof Van Hecke
Dear all, I'm wondering what's the best way to add an extra (tighter) distance restraint on the linkage between the P-atom of a guanidine (Gd)-residue and the O3'-atom of a thymidine (Td)-residue in an oligonucleotide structure, with use of the program Refmac_5.2.0019 (ccp4-6.0.2)...? And i

[ccp4bb] EMBO course announcement

2007-12-07 Thread xavier siebert
Hello, This is a short announcement / reminder about the 2d EMBO course on the Combination of Electron Microscopy and X-ray Crystallography for Structure Determination. Topics cover

[ccp4bb] Post-doc position at Karolinska, Stockholm

2007-12-07 Thread Marjolein Thunnissen
Now the proper version, sorry for that! Post-Doctoral position available Karolinska Insitute, Sweden A post-doctoral position is available at the Department of Medical Biochemistry and Biophysics (MBB), Karolinska Institute, Stockholm. The project deals primarily with overexpression, purificatio

[ccp4bb] Post-doc position at Karolinska, Stockholm

2007-12-07 Thread Marjolein Thunnissen
Post-Doctoral position available Karolinska Insitute, Sweden A post-doctoral position is available at the Department of Molecular Biochemistry and Biophysics, Karolinska Institute, Stockholm. The project deals with overexpression, purification and crystallisation of a receptor involved in inflam