Hi All
i want to display symmetry molecules in COOT, but regularly getting the
following warning:
There are no model molecules that can display symmetry (Cryst1 problem).
How to troubleshoot this Cryst1 problem
thanks in advance
Vineet gaur
Dear All:
Thanks a lot for the prompt replies. The following are the good ideas I have
got so far besides some details that I forgot to mention earlier.
I tried 10 buffers from pH5 to pH9.0 at 5mM with a final protein concentration
of 0.5mg/ml to 1mg/ml. And I also tried to add 0.1M NaCl at
... meant to include this logfile in my previous post:
-Jan
/sw/xtal/ciftr/ciftr-v2.053-prod-bin-darwin/bin/CIFTr -i output.cif
Translating a mmCIF file [1] output.cif to PDB file output.cif.pdb
Warning skipping item _computing.pdbx_density_modification is NOT in
dictionary.
Warning skipping item
Hi all,
has anyone sorted out how to get ciftr running?
pdb_extract nicely bundles the information from the pdb header and
additional information from the supplementary file (data_template.text, for
instance) and writes it into the a cif file.
CIFTr, however, does not re-translate lots of informati
There is also the soluble KcsA.
Computational design of water-soluble analogues of the potassium channel
KcsA. A. M. Slovic, H. Kono, J. D. Lear, J. G. Saven, and W. F. DeGrado
(2004) PNAS 101, 1828-1833
Bil
Bil Clemons, PhD
Assistant Professor of Biochemist
It's hard. See:
J Mol Biol. 2005 May 6;348(3):777-87.
X-ray structure of a water-soluble analog of the membrane protein phospholamban:
sequence determinants defining the topology of tetrameric and pentameric coiled
coils.
Slovic AM, Stayrook SE, North B, Degrado WF.
Slovic, A. M., Summa, C. M.
Hi,
I am wondering whether there is a way to turn a membrane protein with known
crystal structure into a water soluble protein by systematic mutagenesis. I
guess it should be doable if we introduce enough hydrophilic residues on the
surface. Has anyone tested this crazy idea before? Thank
sorry for the repeats.
Jerry
From: [EMAIL PROTECTED]
To: [EMAIL PROTECTED]
Subject: protein induced precipitation of proteins?
Date: Mon, 3 Dec 2007 09:25:51 -0800
Dear All,
Recently I was trying to crystallize a
complex of two proteins(40kD vs 20kD). TO my surprise, precipita
Dear All,
Recently I was trying to crystallize a
complex of two proteins(40kD vs 20kD). TO my surprise, precipitates immediately
appeared in the solution even when I mixed these two proteins at about
1mg/ml. However, the individual proteins were just soluble and stable
in the same buffe
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Dear All,
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Please bring this position to the attention of anyone in your lab who might
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Thanks,
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The Scripps Research Institute
Palm Beach Florida
Post-doctoral Position:
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The Scripps Research Institute has established a ma
Dear All,
We are pleased to announce the release of the Phaser 2.1.1 module as an
update to the CCP4 6.0.2 public release. This replaces Phaser 1.3.3
which was part of the original 6.0.2 release.
Phaser 2.1.1 includes SAD Phasing and Combined MR and SAD Phasing. For
full details, see the include
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