Did you confirm this mutation rigorously? Specifically do the DNA sequencing
results (at least double strand sequencing) and the MS of the purified
protein agree with the expected mutation?
Better be safe than sorry.
Regards,
Artem
-Original Message-
From: CCP4 bulletin board [mailto:[E
Dear Tiancen Hu:
Two things you need to do:
1. Try refining with cis-proline and see if it fits better.
2. Construct Fo-Fc and Fc-Fo maps (coot makes this very easy). See what they
are telling you.
Best of luck,
Bill Scott
On Thu, 18 Oct 2007 09:35:44 +0800
HTC <[EMAIL PROTECTED]> wrote
Hi Steve,
You should definitively follow Jürgen's suggestion and try to solve the
structure. If you could get an idea about the crystal packing it would
help you to optimize your DNA construct. This is often critical in
protein-nucleic acid crystallization. You could try to change the length
of you
W.M. B. wrote:
Dear All:
My protein/DNA complex crystal diffracts to 3.2 A ( in-house source).
It crystalized in 20% PEG 8K, NaCaCo 6.5. I tried to add ATP, CaCl2
and spermidine. The diffaction doesn't improve. Would you please give
me some advice?
Thanks a lot,
Steve
How does
CCP4ers,
We have recently become interested in the use of TLS parameters for
studying protein dynamics. In particular, we would like to generate
an ensemble of conformations along the TLS trajectories to use as a
starting point for protein design. Before jumping into this though,
I'd like to ask
Dear All:
My protein/DNA complex crystal diffracts to 3.2 A ( in-house source). It
crystalized in 20% PEG 8K, NaCaCo 6.5. I tried to add ATP, CaCl2 and
spermidine. The diffaction doesn't improve. Would you please give me some
advice?
Thanks a lot,
Steve
A post-doctoral position for two years is available in Dr. V. Dive's
lab:laboratory in the "Section of Molecular Engineering of Proteins"
from the life Sciences Division of the CEA.
The project deals with the development, on structural-basis, of highly
potent inhibitors of zinc proteases of medica
