Hi Jiamu,
is the high B-value of your protein due to motions, which are not
modeled appropiately ? Which program by the way are you using for
refinement ? Then the TLSMD server might help you here. Monomer or
multimer in the asu ? NCS used, if so checked that they actually follow
NCS and you'
Oddly, I have never had this problem with CCP4's data processing program
ipmosflm.
I reinstalled the same version of linux once in an attempt to cure a disk
arbitration problem and ran into the same issue. You have to get the
vender to send you a new file.
yang li wrote:
> Hi,All,
> Now I a
I will try it soon. If it work, I will paste a sumary.
Thanks
On 4/30/07, Jacqueline Vitali <[EMAIL PROTECTED]> wrote:
Hello,
I think it is because the peptide does not have full occupancy. I do
not know if it is OK to fix occupancy to 0.5 and do the refinement.
That would bring temp factor
Dear All:
I am refining a protein-peptide complex struture at 2.6 angstrom resolution.
The data was obtain from a co-crystal and the wilson B factor of the data is
about 70.
The affinity between protein and peptide is about 10E-7 to 10E-8 molar.
Protein fragment of the structure has a common B fac
Hi,All,
Now I am installing HKL2000 and met a curious problem:
The license cr_info was apllied before, the system is fc6 at that time.
Before received the license,
I reinstalled the system for some reason, now the system is fc4. the install
files and anom.dat file
was copied from other peopl
Thank you all for your suggestions.
Gerard - MAVE worked nicely ... once I remembered how to get data blocks
out of O.
A summary for the future:
I wanted to superimpose electron density maps from two crystal forms of
a protein; the apo protein (P43212) and a crystal into which substrate
was soak
Thank you for the replies. coot 0.3.1 gave me the option of mutating
a pdb file to a semi_polyala (via mutate residue range, no map
required) but the rotamers were changed for the conserved residues.
Chainsaw worked beautifully, however, using the wildtype A.pdb and a
hand-crafted sequence
If I understand you correctly, the CCP4 program Chainsaw would do
this.
It uses an alignment file, which you could hand-craft to select
mutate or conserve.
Keyword "MODE MIXA" to select the truncate-to-beta option.
HTH
Martyn
-Original Message-
From: CCP4 bulletin board on behalf of [EM
[EMAIL PROTECTED] wrote:
Hello everyone,
I have two pdb files for molecule A (A.pdb and A_polyala.pdb) from
which I would like to make a hybrid file A_semi.pdb. A_semi.pdb will
have some residues with full sidechains (so derived from A.pdb) and
some residues with only a C-beta atom for a
POSTDOCTORAL POSITION in protein engineering for nano-material development
This is an interdisciplinary project that aims at the design and production of
tailor-made protein building blocks for the development of biomaterials.
The candidate will be in charge of the molecular design, genetic manip
10 matches
Mail list logo
