Dear all,
Below is a question my friend asked me, but I have never worked on
phosphorylated proteins.
Has anyone worked on crystallizing phosphorylated proteins and can you comment
on it?
Thanks
Rongjin Guan
--
I would like
Crystallization: focus on membrane proteins.
http://www.nsls.bnl.gov/newsroom/events/workshops/2007/crys/
The Crystallization Course at Brookhaven National Laboratory this year will
focus on membrane proteins.
Sponsored by the IUCr the purpose of the course is to provide participants with
hands
We need to run phase combination twice with SigmaA via CCP4i and
encountered some confusion in FOM column label assignment. First, two
sets of MAD phases are combined, we have output figure of merit, WCMB,
from the input FOM_mad1 and FOM_mad2. Second time, we wanted to combine
the Fc and PHIC o
Postdoctoral position in structural immunology
The newly established X-ray crystallography lab of the La Jolla
Institute for Allergy and Immunology (LIAI) has a job opening for a
structural biologist to work on the crystallization and structure
determination of various members of Tumor Necr
Hi Sreeram,
assuming you have plenty of those crystals, why don't you loop a few
pass them through a drop of your reservoir for washing and load them on
a SDS gel ?
Juergen
Sreeram Mahesh wrote:
Hi All!
I have been trying to screen for my protein crystals, from the
crystals grown
There are two positions available for people to join the team developing
Phaser, funded by the NIH grant that supports the development of the Phenix
package. (Of course, the same version of Phaser goes into CCP4 as well!)
If you're interested, please look at
http://www.jobs.ac.uk/jobfiles/ZE33
Third imgCIF workshop (new series) at BSR 2007 in Manchester and at Diamond:
The Management of Synchrotron Image Data:
Changes to the imgCIF dictionary and software, interaction with NeXus
Sponsored by DOE under grant ER64212-1027708-0011962, NSF under grant
DBI-0610407.
You are cordially invit
Multiple overlapping salt lattices can sometimes look like protein
diffraction, as long as you're looking in only two dimensions. However, if
you can find the dominant rings, you should be able to discriminate since
the c-spacing of salt would nearly always be pretty small. Consider powder
patterns
Hello Tiancen,
The pundits often suggest keeping the selenium reduced (and indeed, it's
not a bad idea) however if you're worried about disulphides - I would say
that they take precedence over the selenium. If you work reasonably fast
you should be able to have the best of both worlds - have the s
Dave,
Others have given many excellent suggestions :) I'd like to mention that
to a protein biochemist such as myself, another obvious solution would be
to take an aliquot of the protein for a metal analysis (via ICP or AA).
ICP is more sensitive. Both Mn and Ca will light up the spectrum like new
Hi David,
Like Stephen, I have found that integrating the peaks
tends to remove much of the B-factor dependence of the
analysis seen when looking at the anomalous peak heights.
I too tend to use mapman and typically integrate a sphere of 2.5-3 A
around my anomalous scatters (S, Se, metals). I
Although the peak height of S atoms can be used as an internal yardstick,
one has to worry about differences in occupancy and possibly hetergeneous
sites (i.e. Ca, Mn and Mg) which can confuse the interpretation of the
results.
On Mon, 16 Apr 2007, Eleanor Dodson wrote:
In cases like this I
Hi All!
I have been trying to screen for my protein crystals, from the
crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate
Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6.
Two different kinds of crystals observed: rod shaped and thin platy
ones. Whe
In cases like this I use the S atoms to calibrate the peak height.
Of course it isnt definitive a) it is near the noise level, and b) peak
height is very dependent on B factor..
But the ratio might distinguish between an atom with an f" of 1.3 or f"=2.8
Eleanor
David Briggs wrote:
Dear all.
Team Leader in Structure-Based Drug Design
Institute of Cancer Research, London
The Institute of Cancer Research is seeking to appoint a Team Leader,
who will spearhead the establishment and development of high-throughput
approaches to structure-based drug design within The Institute of Cancer
Res
Hi David,
You can use Sheldrick's Calcium Bond Valence Sum to descriminate
between metals (see Muller, P., Kopke, S., and Sheldrick, G. M. (2003)
Acta Crystallogr., Sect. D: Biol. Crystallogr. 59, 32-37) even at low
resolution. I have had good success with this method combined with
estimation of
There is a bug in the latest imosflm (v0.5.2) which prevents the
correct direct beam coordinates being read from the image header for
Mar 3x3 tiled CCDs (225mm square, as installed on ID23-1 and BM14 at
ESRF for example). This is the ONLY detector affected by this bug.
When the program cannot read
Hey David,
You can do Mn2+ identification by its anomalous diffraction using the Cu
Kalpha radiation. Mn is an anomalous scatterer at Cu Kalpha (1.5418 A),
despite being distant from its absorption edge (somewhere around 1.96 A
if I remember well). I did this for a double-manganese bound ConA,
David Briggs wrote:
Dear all.
I have recently solved a structure in-house, 2.8A, CuKa.
I have a metal ion bound very obvious hepta-valent co-ordination, which
would suggest either Ca or Mn.
Neither was present in the crystallisation setup, but there was some Mg
around, which has contaminants o
Dear all.
I have recently solved a structure in-house, 2.8A, CuKa.
I have a metal ion bound very obvious hepta-valent co-ordination, which
would suggest either Ca or Mn.
Neither was present in the crystallisation setup, but there was some Mg
around, which has contaminants of both Ca & Mn.
At 2.8A
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