Hi Sreeram,
assuming you have plenty of those crystals, why don't you loop a few
pass them through a drop of your reservoir for washing and load them on
a SDS gel ?
Juergen
Sreeram Mahesh wrote:
Hi All!
I have been trying to screen for my protein crystals, from the
crystals grown in 0.5 M Ammonium Sulphate, 1.0M Lithium Sulphate
Monohydrate in 0.1 M TriSodium Citrate Buffer dihydrate Buffer at pH 5.6.
Two different kinds of crystals observed: rod shaped and thin platy
ones. Whenever I am trying to collect data from the rod shaped ones I
am getting dominantly salt patterns but also spots at 15 A resolution
bin repeatedly, where some of the spots very much look like from protein!
what can be the reason for this? if anyone has come across similar
situation please help.
with regards,
Sreeram Mahesh
Research Student
Prof S Ramakumar's lab
PHYSICS department
IISc Bangalore-560 012.
ph:080-2293 2718.
mobile: 9241145183.
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
