What often works for this scenario:
Change temperature. Try 21, 15, and 4C.
Add glycerol or ethylene glycol - set a screen with your condition as basis,
and % EG/glycerol across - from 1% to 15% in suitable increments
Find additives that produce no crystals, set up tray(s) with those, then
se
Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the
condition to improve the size of the crystal and reduce the number of
nucleation.
The crystals are coming from five to six conditions , all have PEG 3350 in
common.
Apart from PEG, t
Re a commensurate superlattice case
Dear CCP4bb Colleagues,
Thanks to the varied interest and especially even as far as browsing and
processing of diffraction images kindly undertaken by Kay Diederichs,
Gloria Borgstahl and Jeff Lovelace, I went back to one-by-one scrutiny of
images including a
Mark,
I suggest you start by doing MR with the twinned data to search
for the individual domains to obtain an initial solution. With near
perfect merohedral twinning, deconvoluting your structure factors is
unreliable. To what resolution does your crystal diffract? If it is
beyond ~2.5Ang.,
Hi,
I'd greatly appreciate advice on how to proceed with trying to solve and refine
a structure with
nearly perfect merohedral twinning - or is this impossible? The SG is H3; most
likely nmol is asu
is 4 from Matthews coefficient - but not sure about this.
Here are the twinning stats from p
This is a replacement for cns2mtz, which fixes all the reported bugs in
that program, is rather more convenient, and handles other ascii file
types as well, including at least SHELX hkl files and XtalView PHS files.
Changes include:
- Spacegroup headers are now generated correctly. (I hope).
