Re: [ccp4bb] AKTA prime

Dear Frank AKTAs are excellent chromatographic systems, but you must know that AKTA prime is not the best choice if you want to do "serious" chromatography. AKTA prime has only one pump and a valve that alternatively extract solvent from the two different reservoirs. If you want to do a gradient,

Re: [ccp4bb] AKTA prime

Dear Frank AKTAs are excellent chromatographic systems, but you must know that AKTA prime is not the best choice if you want to do "serious" chromatography. AKTA prime has only one pump and a valve that alternatively extract solvent from the two different reservoirs. If you want to do a gradient,

Re: [ccp4bb] AKTA prime

Are you sure that you want AKTA anything? They are so expensive. We have had good service from the old style ("HR") Bio-Rad Bio-Logic chromatography systems. The new ones are called "DuoFlow". -Dan On Tue, 13 Feb 2007, Frank Lee wrote: > > Dear all, > > I need to decide between buying an AKTA p

Re: [ccp4bb] Multi-copies in one assymetric unit

Hi, Do you have any biochemical evidence that points to oligomeric nature of your protein? Does SEC indicate monomer/dimer/trimer/tetramer? If not or even if so, what does the crystal packing with your MR solution with 4 molecules suggest about monomer interactions? Does that correlate with S

Re: [ccp4bb] Multi-copies in one assymetric unit

Sorry I forget to tell ya the details: The SG of my Data is cubic P4332. Cell is 251.34A in all three dimensions. Resolution is 3.1A. 5,6,7,8 might be the possible copies from Matthew coeficient. But I just trust 6 because Chinese like the number 6 (happy Chinese new year by the way :)). No pseud

[ccp4bb] AKTA prime

Dear all, I need to decide between buying an AKTA prime and an AKTA FPLC from GE health care. I understand AKTA prime is a low-pressure system, but because it is too much cheaper than AKTA FPLC, it is still very attractive to me. I will mainly use it for Nickel columns and gel filtration colum

Re: [ccp4bb] Structure reconstruction from NMR constraints?

> software is recommended for building structures Actually, I can recommend some HARDWARE: It is called 'X-ray diffractometer'. Cheers, BR -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Dan Bolser Sent: Tuesday, February 13, 2007 4:54 AM To: CCP4BB@J

Re: [ccp4bb] Multi-copies in one assymetric unit

Assuming that the MR solution is correct (I agree with Eleanor, that you need to be certain about your selection of space group), then use your complete model of chain A superimposed on chains B, C, and D. Then refine and build the heck out of it. In a similar situation, it was relatively easy for

Re: [ccp4bb] Multi-copies in one assymetric unit

Sometimes this sort of disorder is due to an error , so the first thing is to check very carefully that the solution makes sense. Why are you so sure there are 6 copies in the asymmetric unit? In situations like this I first worry about SG. Is there a pseudo-translation vector? This can make i

[ccp4bb] REPOST: Post-doc at Colorado State

This is merely a second posting of a message from about 10 days ago - there is no need to reply if you have already expressed interest

Re: [ccp4bb] molrep

Is the result clearly better in one space group than all the others? ie Are you sure you have a correct solution? Eleanor shivesh kumar wrote: Dear all I am trying MR in molrep(CCP4) with P21212.mtz.I have tried nearly all space group.Rfact and corr.coeff is 57-59% and 23-25% resp.what shoul

[ccp4bb] molrep

Dear all I am trying MR in molrep(CCP4) with P21212.mtz.I have tried nearly all space group.Rfact and corr.coeff is 57-59% and 23-25% resp.what should I try to lower the Rfact.The data is at 2.2A.Any suggestions are welcome.Thanx in advance. Shivesh

[ccp4bb] Structure reconstruction from NMR constraints?

Hi, What software is recommended for building structures from NMR data? Cheers, Dan.

[ccp4bb] Multi-copies in one assymetric unit

Dear All, Maybe this is a trivial question: My data should have 6 molecules in one assymetric unit. MR could find out 4 molecules. After this, no matter how hard I have tried, no more molecules can be found. At this stage, I suppose that all other copies are dis-ordered. And go ahead to do r

[ccp4bb] call for proposals for beamtime at BM14/ESRF for APR/MAY 2007 RUN

Dear all, its easy and paperless, so get your proposals in for the next round of beamtime at bm14. See links below for submitting your proposals SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT THE UK/EMBL MAD

[ccp4bb] Beamtime at SLS X06SA

= SYNCHROTRON BEAM TIME FOR MACROMOLECULAR CRYSTALLOGRAPHY AT THE UNDULATOR BEAMLINE X06SA AT SLS FROM MAY - AUGUST 2007 = - PILATUS 6M pixel detector will become ava