Hi,
I have a basic operational problem. I am trying to convert a pair of
TNT HKL format files into an MTZ file using the CCP4I interface. (I realize
this is probably not the most heavily exercised code in the package.)
When I run the task I get the following output.
#CCP4I VERSION CCP4Interf
Hi Rebecca
You will be able to find the dictionary entry for BOG (B-octylglucoside) on
the HIC-UP server, located at http://xray.bmc.uu.se/hicup/
Search for your molecule (I looked for BOG and found it listed under Hetero
compounds sorted by residue type name) and find the dictionary entry that
yo
Hi Bill,
This sounds very unusual. We have 3rd party RAM in almost all of our
Macs, and I've never had a repair request denied by AppleCare. This
is an allowed DIY (do it yourself) installation in most if not all
their recent machines. It's common knowledge now that hard drives
fail al
CCP4ers:
I am trying to use coot (and refmac) to model and refine a structure
with b-octylglucoside.
The .cif library file in my ccp4 data directory for b-octylglucoside
(BOG.cif) does not contain restraints. How can the restraints be
generated? Does someone already have such a file for BOG
It's Apple itself. It was no longer under warranty. They went to the
trouble to pull out the memory and put it in an envelope separately with
the message.
Needless to say, I will make sure I pull out any 3rd party memory before
sending anything to them again.
Serge Cohen wrote:
> -BEGIN PG
-BEGIN PGP SIGNED MESSAGE-
Hash: SHA1
Hi Bill;
Given the fact that for laptops apple puts the instructions on how to
add/change the RAM in the user manual. Given also the fact that the
position of the RAM and the disk are far apart it looks like the
person servicing your laptop was
Hi folks:
Sorry this is a wee bit off topic, but since I am more likely to get
a straight answer from people here, I'm going to ask..
I have 2 identical laptops. We bought both for the lab about 2 years
ago. They are G4 ppc. I bought an extra half gig of memory for each
at the time of
No just common buffer substances: Tris, NaCl.
I think the Nitrogen I used for pressure filtration is solved. It took
a long time, the pressure was high (I think around 5 bar) and I was
stearing it.
Since then it was was in an NMR tube with an stamp on the fluid so that
I noticed every bubble.
Thank you for all the replies.
@ Kris
It is not the problem of single gas bubbles, but of solved gas. I
prepared the sample in the middle of December and it still degases that
means bubbles appear. They can be removed but after a few days new ones
are coming up. I'm doing NMR and the shimming
Put it in 1 ml syringe, seal the end with your finger (use a piece of
parafilm in between if you need it) and then draw out the plunger to
create negative pressure, and your bubbles will go away. Then let it
gravity-drain or very slowly push the contents into an eppindorf tube.
or
Speed-vac it
CCP4 bulletin board wrote on 02/08/2007 06:34:44
AM:
> Thanks for the links to the boards!
>
> But I think you could also answer my question. I need a way to remove
> gas, solved in my protein sample after using pressure filtration. My
> problem is the small sample volume of 400 mikroliters.
>
>
On Thursday 08 February 2007 12:34, Justin Schmitz wrote:
> But I think you could also answer my question. I need a way to remove
> gas, solved in my protein sample after using pressure filtration. My
> problem is the small sample volume of 400 mikroliters.
Our microcalorimeters came with a degass
Thanks for the links to the boards!
But I think you could also answer my question. I need a way to remove
gas, solved in my protein sample after using pressure filtration. My
problem is the small sample volume of 400 mikroliters.
greets Justin
P.s. special thanks to James for his construct
Hi Justin,
There is a similar bulletin board for NMR related questions and you can
join the list @ http://www.jiscmail.ac.uk/archives/nmrgen.html
Cheers
Ravi
Justin Schmitz wrote:
Morning every body!
Does anybody know a board for NMR releated questions which has a
quality like this?
Thx
Does anybody know a board for NMR releated questions which has a quality like
this?
when i was a lad, i used to subscribe to one called [EMAIL PROTECTED] -
not sure what its current status is though
--dvd
**
Perhaps we need an extra PDB record (ILLDEF - for ill-defined )
this exists already - CAVEAT
--gerard
**
Gerard J. Kleywegt
[Research Fellow of the Royal Swedish Academy of Sciences]
Dept. of Cell &
Hey Justin,
Try the ccpn board - the same as ccp4 but for nmr. You can subscribe to
this through:
http://www.ccpn.ac.uk/
Hope this helps,
MARKX
_
Dr Mark Agacan
Scientific Officer,
Division of Biological Chemistry
and Molecular Microbiology,
Wellcome Trus
There is an NMR Board. Problem is that you have to abide by its Rules:
First Rule: You do not talk about the NMR board.
Second Rule: You DO NOT talk about the NMR board.
James
Justin Schmitz wrote:
Morning every body!
Does anybody know a board for NMR releated questions which has a
qualit
I guess you can use this one - it certainly isnt restricted to
crystallographic Qs..
Eleanor
Justin Schmitz wrote:
Morning every body!
Does anybody know a board for NMR releated questions which has a
quality like this?
Thx for your answers
Morning every body!
Does anybody know a board for NMR releated questions which has a quality
like this?
Thx for your answers
I agree you should describe, and deposit what you have along with the
experimental data.
Then if anyone really wants to examine the structure in more detail,
they can.
Perhaps we need an extra PDB record (ILLDEF - for ill-defined )
Eleanor
Richard Gillilan wrote:
I'm sure many of you have bee
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