Hi,
Sorry to bother you all.I'm going to try to reproduce some results
from the initial screening from PACT kit.The conditions are something
like 0.1M PCB buffer, 25% w/v PEG 1500 and 0.1 MMT buffer, 25%w/v PEG
1500. I was just wondering is there any easy way to get these
buffers?Do you happen to
Here's another late addition to the discussion, which Chad's comment
reminded me
of:
We just deposited a similar-sounding structure of a DNA polymerase
(2HVI). The
protein is in the closed conformation, with ddCTP pairing with G in the active
site of the two monomers in the ASU. A third ddCT
Hi, Phoebe,
Sorry to jump in late on this one- but I second Stefan's note here. When
soaking dinucleotides (which are poor substrates) into Klenow Fragment xtals, I
noted binding both at the active site and at a crystal interface site that is
likely nonphysiological. The adventitious site is
I have a GRASP session saved which I startup by reading in "grasp.state". I
have changed some colours in "defcol.dat" and I would like these changes to
take effect in my saved session, but everytime I read in "grasp.state" it
changes the colour palette back to the default set again.
Does anyone kn
You can use any acronym and your dictionary file overrides any existing
entry.
but check at the RSCB or EBI if your ligand already has a defined ID.
Isnt a dsn6 file a map file, whereas an mtz file is a list of reflections?
You can calculate a CCP4 map from your mtz file, then read that into O
Thanks a lot for the replies. The option which allows to set the chirality
restraints to "both" was exactly what i was looking for, since i use refmac for
refinement. Surprisingly, the binding site seems not to be stereoselective in
this case.
greetings, david
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