Hi all,
yesterday i have tried annealing, no significant improvement.
Thank you so much for all those suggestions.
Summary:
1. check diffraction on RT.
2. dehydrate crystal, many methods
3. try annealing
4. grow crystal in presence of cryo
5. try smaller crystal, sometimes small crystal is bette
Dear All,
Could you please help me solve this problem?
I have a sensitive crystal, the mother liquor is 10% PEG4k+ 100 mM
Tris-buffer pH 8.5, crystal is big and good but very sensitive, when i put
it in cryoprotectants, cracking happened. First i used artificial mother
liquor + 25% v/v glycerol,
