Hi all, yesterday i have tried annealing, no significant improvement.
Thank you so much for all those suggestions. Summary: 1. check diffraction on RT. 2. dehydrate crystal, many methods 3. try annealing 4. grow crystal in presence of cryo 5. try smaller crystal, sometimes small crystal is better 6. change loop. but my crystal is not thin plate and needle. I will try one by one next week, when machine is available to me, and let you know the result. Thanks again. Fengxia
