[ccp4bb] PhD position available in Structural Biology of Type 4 Secretion Systems at Umeå University, Sweden

[Ronnie Berntsson]

Dear colleagues,

We have a PhD student position available, for structural and functional studies 
of bacterial Type 4 Secretion Systems.
We are looking for an enthusiastic and highly motivated individual who has an 
interest in large protein machineries. We use biochemistry, molecular biology, 
x-ray crystallography and cryo-EM to study Type 4 Secretion Systems, mainly of 
Gram-positive origin.

The Berntsson lab is part of the integrated structural biology network at Umeå 
University, and from the 1st of December also part of the Wallenberg Center for 
Molecular Medicine. We are well equipped with state of the art technologies, 
both for crystallography and EM, including a nanodrop crystallisation robots, 
Formulatrix crystallisation hotel, an EM facility with Titan Krios and Scios 
Dualbeam for FIB-milling, as well as other strong facilities.

For further information, and to apply, please go to the official ad:
https://www.umu.se/en/work-with-us/open-positions/phd-studentship-_234429/?fbclid=IwAR0vPOQH8GGTvSLfJxO-cl0fq2CFQv4chtNS4x1hb-_EhzI_e476DmsIqCM

Informal inquiries about the position can be directed to me at 
ronnie.bernts...@umu.se. Applications must be 
sent in via the online recruitment system, with a deadline at 7th of December.


Regards,
--
Ronnie Berntsson, PhD
Assistant Professor
Department of Medical Biochemistry and Biophysics
Umeå University
90187 Umeå
Sweden

e-mail: ronnie.bernts...@umu.se
web: https://www.biostruct.umu.se/principal-investigators/ronnie-berntsson/






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[ccp4bb] cryo-EM position at ILL/PSB, Grenoble, France - application deadline on 4 November

[Florent Bernaudat]

Dear all, 

a Tenure-track Research Lectureship in Cryo-Electron Microscopy (Fixed
term for 5 years) is currently available at the Institut Laue Langevin,
Grenoble, France. Deadline for application is 4 NOVEMBER. 

https://www.jobs.ac.uk/job/BMR152/tenure-track-research-lectureship-in-cryo-electron-microscopy-joint-appointment-with-institut-laue-langevin-Grenoble


Please distribute to anyone who may be interested. 

Best regards, 

Florent

-- 

Florent Bernaudat, PhD
PSB Scientific Coordinator/Animator

Partnership for Structural Biology
Carl-Ivar Brändén Building- office 018
71, Avenue des Martyrs, 
CS 90181
38042 Grenoble cedex 9
France

http://www.psb-grenoble.eu
Phone: 33 (0) 476 20 94 08
Fax: 33 (0) 476 20 94 00



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[ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4

[Almudena Ponce Salvatierra]

Dear all,

I have looked into the archive and I've tried to collect suggestions for
cryo protecting crystals that grew in 1.8 M Lithium sulphate containing
mother liquor or 2M.

What would make more sense? Transferring it into a cryo solution that
consists of mother liquor and a higher concentration of Lithium sulphate?

Or, would this concentration of Lithium sulphate be enough as a
cryo-protectant and after slight dehydration I could flash freeze them?

Thank you very much in advance.

Best

Almu



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[ccp4bb] Postdoctoral position available at Structural Biology Unit, IBMB-CSIC, Barcelona

[Laia Vives Adrián]

Dear all,

a 3-year postdoctoral position at the Structural Biology Unit (IBMB-CSIC,
Barcelona) is available.

Find all the information at the following link
:

https://sbu.csic.es/jobs/postdoctoral-position-gomis-ruth-2018/

Please distribute to anyone who may be interested.

Laia Vives Adrián
Project Manager & Head of Communication

Structural Biology Unit (IBMB-CSIC)
Parc Científic de Barcelona
c/Baldiri Reixac, 15 08028 Barcelona
Tel: 934020189

www.sbu.csic.es
 @sbu_csic 
 Structural Biology Unit CSIC




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[ccp4bb] Open PhD position, MFPL Vienna

[Falk, Sebastian]

PhD Position at MFPL Vienna
I will start my group at the Max F. Perutz Laboratories (MFPL) in Vienna in 
March 2019. I am currently recruiting a PhD student through the Vienna 
BioCenter PhD Programm (see below).
Note: Only application through the Vienna BioCenter PhD program can be 
considered. The application deadline is November 15th.
Project: My group is interested in unraveling the molecular mechanisms that 
allow the establishment of a robust RNA interference (RNAi) response in which 
small RNAs are used to silence the expression of genes. We are particularly 
interested in understanding how the nuclear RNAi pathway leads to 
heterochromatin formation. We will address these questions by reconstituting 
and characterizing the protein complexes involved in these pathways both 
biochemically and structurally. For the structural characterization, we employ 
an integrative approach by combining X-ray crystallography and single-particle 
electron microscopy. This will be complemented by biochemical approaches 
(enzyme activity assays) and biophysical assays (isothermal titration 
calorimetry, analytical ultracentrifugation, and spectroscopic methods). For 
more information see the link below:
https://www.training.vbc.ac.at/fileadmin/user_upload/SebastianFalk_VBC_WinterSection_18_19.pdf
Best,
Sebastian
––
Dr. Sebastian Falk
Max-Planck-Institute of Biochemistry
Structural Cell Biology / Conti Lab
Am Klopferspitz 18
D-82152 Martinsried
Germany

 Apply now to one of the best PhD Programmes in Europe!
International PhD Programme in Molecular Life Sciences at the Vienna 
BioCenter
Empowering Curious Researchers
The Vienna BioCenter (VBC) is a leading life science location in Europe: a 
thriving campus that combines basic research, universities and biotech 
companies. Our programme has been successfully running since 1994, providing 
students from all over the world with exceptional training. As a student at the 
VBC you will be supervised by top scientists and have full access to 
state-of-the-art facilities, thus giving you the opportunity to launch a 
successful scientific career.
At the Vienna BioCenter you can do more, as we offer:

  *   Fully-funded PhD Positions with competitive salaries

  *   A multidisciplinary environment and full access to state-of-the-art 
facilities
  *   A dynamic and collaborative scientific environment at the Vienna 
BioCenter.
  *   English as the working language
  *   A flexible curriculum with an initial training course, progress reports, 
as well as training in technical and complementary skills.
  *   Students contribute to the management of the institutes, through 
representatives, and organize events that promote networking and career 
development, such as an annual retreat and a symposium.
  *   Not only will you be in one of Europe’s best institutes, you will live in 
Vienna, which is rated as the number one city in the world for quality of life. 
Vienna is a fun, young city embedded in beautiful old buildings, an 
international metropolis in the heart of Europe.

The campus:
Four research institutes collaborate in creating an exceptional environment, 
where researchers have all the necessary resources to conduct meaningful 
projects: there is virtually no experiment that cannot be done at the VBC. The 
VBC PhD Programme is jointly organized by: Research Institute of Molecular 
Pathology (IMP): www.imp.ac.at, Institute of Molecular 
Biotechnology (IMBA): www.imba.oeaw.ac.at, Gregor 
Mendel Institute (GMI): www.gmi.oeaw.ac.at, Max F. 
Perutz Laboratories (MFPL): www.mfpl.ac.at

Eligibility criteria:
The programme is run in collaboration with the University of Vienna. Applicants 
must hold a Master's degree or equivalent from a university in a related topic 
(or you will complete a master's degree within six months of the application 
deadline). We have two calls per year
How to apply:
We have two calls per year: Winter call between 15 September – 15 November; 
Summer Call between 1 March – 15 April
All applications must be submitted online: 
https://www.training.vbc.ac.at/phd-programme/applications/
Visit our website or our social media channels for more information and updates:
https://www.training.vbc.ac.at/
https://www.facebook.com/VBCPhdProgramme/
https://twitter.com/TrainingVBC
https://www.youtube.com/channel/UC4UetFcTZbVyCet7sJ7lIqg?disable_polymer=true





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[ccp4bb] Job Offer: Group Leader in Structural biology or Biochemistry

[Fan Jiang]

Dear colleagues, 

Viva Biotech, located in Shanghai, China, is specialized in structure-based 
drug discovery providing preclinical drug discovery research services to global 
pharmaceutical and biotech companies. We have opening for group leader in 
structural biology and biochemistry. Anyone who is interested in the position  
please send CV to jin@vivabiotech.com.

Job title: Group Leader in Structural Biology or Biochemistry

Job description

We are seeking a highly motivated individual with experience in protein 
biochemistry and structural biology. Candidates will lead the structural 
biology team to carry out the biochemistry studies and/or gene-to-structure 
studies which elucidate the interactions between small molecules/peptides and 
their targets. Experiences with protein expression and purification, excellent 
interpersonal and managerial skills, team player with excellent written and 
verbal communication skills and proficiency in structure biology related 
software. Excellent communication skill in English; 3-5 years working 
experiences with in US or Europe.

Qualifications

1)  Ph.D. in crystallography or protein biochemistry training with minimal 
3-5 years’ working experience 

2)  Hands-on experience in protein crystallization, and protein expression 
and purification is optional. 

3)  Proficient in structure biology related software is plus. 

4)  Demonstrated managerial skill to guide groups through corporate change 
and growth. 

5)  Excellent verbal and writing skills in English are essential.

6)  Strong communication skills and client interaction are required.



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Re: [ccp4bb] [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4

[Mooers, Blaine H.M. (HSC)]

Hi Almu,

In my hands, 2M LiSO4 is not enough.

If your crystals cannot survive transfer to higher concentrations, transfer 
them to 1.8 M NaMalonate at the same pH and then transfer or add aliquots of Na 
Malonate stock at higher concentration until the drop has about 3.5 - 3.8 M Na 
Malonate. The optimal final concentration may vary from system to system. This 
protocol has worked for us many times.

Best regards,

Blaine

Blaine Mooers, Ph.D.
Associate Professor
Department of Biochemistry and Molecular Biology
College of Medicine
University of Oklahoma Health Sciences Center
S.L. Young Biomedical Research Center (BRC) Rm. 466
975 NE 10th Street, BRC 466
Oklahoma City, OK 73104-5419

office: (405) 271-8300   lab: (405) 271-8313
e-mail:  blaine-moo...@ouhsc.edu 
alternate e-mail: bmooe...@gmail.com 

Faculty webpage: https://tinyurl.com/BMBmooers
Google Scholar: https://tinyurl.com/GSbmooers
SSRL User Executive Committee: 
https://www-ssrl.slac.stanford.edu/content/about-ssrl/advisory-panels/ssrl-users-organization
SSRL-LCLS User Meeting: https://conf.slac.stanford.edu/ssrl-lcls-2018/
EasyPyMOL: https://github.com/MooersLab
Molecular Graphics Course Links: https://tinyurl.com/molgr
Small Angle Scattering Links: https://tinyurl.com/ouhscsaxs
X-ray lab (LBSF): https://tinyurl.com/ouhsclbsf

From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Almudena Ponce 
Salvatierra [maps.fa...@gmail.com]
Sent: Tuesday, October 30, 2018 6:44 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition 
containing 1.8 - 2 M Li2SO4

Dear all,

I have looked into the archive and I've tried to collect suggestions for cryo 
protecting crystals that grew in 1.8 M Lithium sulphate containing mother 
liquor or 2M.

What would make more sense? Transferring it into a cryo solution that consists 
of mother liquor and a higher concentration of Lithium sulphate?

Or, would this concentration of Lithium sulphate be enough as a cryo-protectant 
and after slight dehydration I could flash freeze them?

Thank you very much in advance.

Best

Almu



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[ccp4bb] Postdoctoral position at LNBio (Campinas, Sao Paulo, Brazil)

[Daniel Maragno Trindade]

We are opening one postdoctoral position at the Brazilian Biosciences National 
Laboratory - LNBio (http://lnbio.cnpem.br/) in Campinas – Sao Paulo in Dr. 
Andrea Dessen’s group. The position involves: (1) fractionation and 
purification of bacterial secondary metabolites, as well as (2) testing for 
molecule recognition of bacterial cell wall synthesis proteins via 
crystallography. The successful candidate could start in the post during the 
first semester of 2019. The contract is for two years, and there is the 
possibility of an extension.
Candidates should have a recent PhD degree Biochemistry, Structural Biology, 
Chemistry or related disciplines and have published at least one research 
article in an international peer-reviewed journal as first author. The 
successful candidate should have practical knowledge in biochemistry and 
protein structure determination. Demonstrated expertise in one of the following 
areas would be advantageous: natural product purification and chromatographical 
analysis methods, and proteins expression and purification. Applicants are 
asked to submit a CV including a list of publications, a short description of 
previous experience and names of references to Dr. Andrea Dessen 
(andrea.des...@lnbio.cnpem.br) by November 30th, 2018.

--

Estamos abrindo uma vaga para pós-doutorado no Laboratório Nacional de 
Biociências – LNBio (http://lnbio.cnpem.br) em Campinas, São Paulo, Brasil no 
grupo da Dra. Andréa Dessen. A vaga abrange: (1) o fracionamento e purificação 
de produtos de metabolismo secundário de bactérias, bem como (2) a realização 
de ensaios de ligação de moléculas originárias destas frações com proteínas 
envolvidas na síntese da parede bacteriana por cristalografia. O(A) 
candidato(a) deverá iniciar na vaga no primeiro semestre de 2019. A bolsa tem a 
extensão de dois anos podendo ser estendida por mais um ano.
Os candidatos devem possuir um título de doutor recente na área de bioquímica, 
biologia estrutural, química ou área correlata e ter publicado ao menos um 
artigo científico em revista cientifica indexada como primeiro autor. O 
candidato deve ainda possuir conhecimento prático (hands-on) em bioquímica e 
determinação de estrutura de proteínas. Experiência comprovada em uma das áreas 
a seguir é desejável: métodos de purificação e análise cromatográfica de 
produtos naturais, expressão e purificação de proteínas. Os interessados devem 
submeter um CV contendo: lista de publicações, breve descrição de sua 
experiência prévia e nomes de referências para Dra. Andrea Dessen 
(andrea.des...@lnbio.cnpem.br) até dia 30 de novembro de 2018.

--






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[ccp4bb] Winter School of Cryo-EM Workshop at Arizona State University (ASU)

[Po-Lin Chiu]

Dear everyone,

We are pleased to announce the Cryo-EM Workshop of the High-Resolution TEM
Winter School, which will be held on December 11-14, 2018 at Arizona State
University (ASU) Tempe campus.  The HRTEM Winter School at ASU has a long
history in educating and training TEM scientists, and we have been
expanding our interests and courses to the biological applications,
especially the cryo-EM methods.

The goal of the Cryo-EM Workshop is to introduce the concepts and
applications of the biological cryo-EM.  We will have four guest speakers
coming to join our workshop: Dr. James Evans (Pacific Northwest National
Laboratory), Dr. Tim Grant (HHMI Janelia Farms), Dr. Axel Brilot (UCSF),
and Dr. Thomas Tomasaik (University of Arizona).  Participants will learn
the complete workflow and advanced techniques used for biological cryo-EM
through lectures, demos, and hands-on experiments.  No previous experience
is required.  Also, it will be a great opportunity for the participants to
learn the cryo-EM principles while working along with their samples.
Therefore, we are welcoming to receive the proposals from who are
interested in bringing their samples and processing their data on site.

More information can be found in https://le-csss.asu.edu/cryoworkshop.
Winter is the wonderful season to visit Pheonix area, and we are looking
forward to welcoming you.  Please don't hesitate to contact us if you need
more information.

Date:  December 11-14, 2018
Place: Arizona State University Tempe campus
URL:   https://le-csss.asu.edu/cryoworkshop

Organization committees: Dr. Brent Nannenga (brent.nanne...@asu.edu), Dr.
Po-Lin Chiu (plc...@asu.edu), and Dr. Dewight Williams (
dewight.willi...@asu.edu).


-- 
Po-Lin Chiu, Ph.D.
School of Molecular Sciences | The Biodesign Institute
Arizona State University
1001 S McAllister Avenue
Biodesign Building A230A
Tempe, AZ 85287
(O) 480.727.2058 | (G) 510.859.3597
https://plchiulab.github.io/
plc...@asu.edu | c...@crystal.harvard.edu



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