Hi Almu, In my hands, 2M LiSO4 is not enough.
If your crystals cannot survive transfer to higher concentrations, transfer them to 1.8 M NaMalonate at the same pH and then transfer or add aliquots of Na Malonate stock at higher concentration until the drop has about 3.5 - 3.8 M Na Malonate. The optimal final concentration may vary from system to system. This protocol has worked for us many times. Best regards, Blaine Blaine Mooers, Ph.D. Associate Professor Department of Biochemistry and Molecular Biology College of Medicine University of Oklahoma Health Sciences Center S.L. Young Biomedical Research Center (BRC) Rm. 466 975 NE 10th Street, BRC 466 Oklahoma City, OK 73104-5419 office: (405) 271-8300 lab: (405) 271-8313 e-mail: blaine-moo...@ouhsc.edu <mailto:blaine-moo...@ouhsc.edu> alternate e-mail: bmooe...@gmail.com <mailto:bmooe...@gmail.com> Faculty webpage: https://tinyurl.com/BMBmooers Google Scholar: https://tinyurl.com/GSbmooers SSRL User Executive Committee: https://www-ssrl.slac.stanford.edu/content/about-ssrl/advisory-panels/ssrl-users-organization SSRL-LCLS User Meeting: https://conf.slac.stanford.edu/ssrl-lcls-2018/ EasyPyMOL: https://github.com/MooersLab Molecular Graphics Course Links: https://tinyurl.com/molgr Small Angle Scattering Links: https://tinyurl.com/ouhscsaxs X-ray lab (LBSF): https://tinyurl.com/ouhsclbsf ________________________________ From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] on behalf of Almudena Ponce Salvatierra [maps.fa...@gmail.com] Sent: Tuesday, October 30, 2018 6:44 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [EXTERNAL] [ccp4bb] cryo suggestions for lithium sulphate xt condition containing 1.8 - 2 M Li2SO4 Dear all, I have looked into the archive and I've tried to collect suggestions for cryo protecting crystals that grew in 1.8 M Lithium sulphate containing mother liquor or 2M. What would make more sense? Transferring it into a cryo solution that consists of mother liquor and a higher concentration of Lithium sulphate? Or, would this concentration of Lithium sulphate be enough as a cryo-protectant and after slight dehydration I could flash freeze them? Thank you very much in advance. Best Almu ________________________________ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.jiscmail.ac.uk_cgi-2Dbin_webadmin-3FSUBED1-3DCCP4BB-26A-3D1&d=DwMFaQ&c=VjzId-SM5S6aVB_cCGQ0d3uo9UfKByQ3sI6Audoy6dY&r=k0gMbcsdOcdbPUNV5tW66KQSZfXL0ewVDPVBp7tqbks&m=UhcylWwlcufpcF4Jiv9Dy7IwlIWVNuQ1wP5I8uUFr04&s=d92INOuMfDBtY9c9rFVaVpHozWUaoRA9ha6coy-umcI&e=> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
