Re: [ccp4bb] Reference for hanging drop crystallization

[mesters]

A very good source for finding such (and other) information concerning 
crystallization of macromolecules is:


Richard Giege, a historical perspective on protein crystallization from 
1840 to the present day, FEBS Journal 280 (2013) 6456–6497 !


Vapour diffusion (in this case sitting drop) was first 
mentioned/reported by Hampel A, Labanauskas M, Connors PG, Kirkegard L, 
RajBhandary UL, Sigler PB & Bock RM (1968) Single crystals of transfer 
RNA from formylmethionine and phenylalanine transfer RNA’s. Science 162, 
1384–1387.


Either use the review as a reference or for the hanging drop version of 
the vapour technique use:


McPherson, A. (1976) Methods Biochem. Anal. 23, 249-345
or
McPherson A. Crystallization of biological macromolecules. Cold Spring 
Harbor, NY: Cold Spring Harbor Laboratory Press; 1999.
(or McPherson A. Preparation and Analysis of Protein Crystals. New York: 
Wiley, 1982).


Best

Jeroen

Am 17.03.18 um 07:00 schrieb Shane Caldwell:

Hi ccp4bb,

I've been asked to provide a reference for making hanging-drop 
crystals, and I suppose that I never realized the need to cite the 
technique specifically. Is there a definitive publication where one 
could trace the origins of the technique, or a particularly thorough 
textbook or review?


Shane Caldwell
shane.caldwel...@gmail.com 

--
Dr.math. et dis. nat. Jeroen R. Mesters
Deputy, Senior Researcher & Lecturer
Program Coordinator /Infection Biology/ 



Institute of Biochemistry, University of Lübeck
Ratzeburger Allee 160, 23538 Lübeck, Germany
phone: +49-451-31013105 (secretariate -31013101)
fax: +49-451-31013104

http://jobs.zeit.de/image-upload/logo_10564.jpg
http://www.biochem.uni-luebeck.de 
http://www.eine-stadt-sieht-gelb.de 
http://www.uni-luebeck.de/studium/studiengaenge/infection-biology
http://www.iobcr.org 

Visiting Professorship in Biophysics, University of South Bohemia (CZ)
President of the International Organization for Biological 
Crystallization (IOBCr)

--
If you can look into the seeds of time and tell which grain will grow 
and which will not, speak then to me who neither beg nor fear 
(Shakespeare's Macbeth, Act I, Scene 3)

--
"Aujourd'hui je sais qu'il n'y a pas de limites à la bêtise humaine - 
qu'elle est infinie." (Gustave Flaubert, French novelist, 1821-1880)

--
It is invariably the case that high resolution X-ray structures show 
significantly better agreement with solution observables such as 
coupling constants, 13C chemical shifts, and proton chemical shifts, 
than the corresponding NMR structures, including the very best ones. 
Hence, in most cases, a high-resolution crystal structure (< 2.0 Å)will 
provide a better description of the structure in solution than the 
corresponding NMR structure  (Kuszewski, Gronenborn & Clore, 1996, 
Protein Science 5:1067-80)

--
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Re: [ccp4bb] Reference for hanging drop crystallization

[Gerard Bricogne]

Dear Jeroen and Shane,

 I am quoting from memory only, as I am not as thoroughly familiar
with the crystallisation literature as I would wish to be, but I can
remember David Blow (with whom I started my PhD in 1972) telling me
that, as far as he was aware, the hanging drop vapour diffusion method
was invented by Robert W. (Bob) Hartley, who used it to crystallise a
small protein called Barnase (Bacillus amyloliquefaciens RNAase) that
later acquired fame through its use by Alan Fersht's group as a guinea
pig for protein engineering. In fact Bob Hartley was visiting the
MRC-LMB at the time I went there, and left behind a stock of barnase
crystals from which the structure was later solved (Nature 1982 May
13;297(5862):162-4) using a single gold cyanide derivative and the
first instance of NCS averaging by an "improper" NCS.

 This recollection was revived a couple of decades later when I
met David again. He told me that he was in the process of nominating
Bob Hartley for an award, in an attempt (unsuccessful AFAIK) to obtain
some degree of recognition from the community for this ground-breaking
innovation that was never published as such and was somehow taken for
granted once it was adopted - perhaps because it was such a brilliant
idea that it didn't need an inventor at all :-) .

 Apologies if this anecdote turns out to overlook someone else's
earlier invention of the technique: I thought I would simply mention
it "as is", although I have a strong belief that David Blow would have
done his "due diligence" before writing such a nomination. If it is
indeed accurate, it would imply that there is no primary citation for
the actual invention of the technique other than "R.W. Hartley (1972,
unpublished results)".


 With best wishes,
 
  Gerard.

--
On Sat, Mar 17, 2018 at 12:02:39PM +0100, mesters wrote:
> A very good source for finding such (and other) information concerning
> crystallization of macromolecules is:
> 
> Richard Giege, a historical perspective on protein crystallization from 1840
> to the present day, FEBS Journal 280 (2013) 6456–6497 !
> 
> Vapour diffusion (in this case sitting drop) was first mentioned/reported by
> Hampel A, Labanauskas M, Connors PG, Kirkegard L, RajBhandary UL, Sigler PB
> & Bock RM (1968) Single crystals of transfer RNA from formylmethionine and
> phenylalanine transfer RNA’s. Science 162, 1384–1387.
> 
> Either use the review as a reference or for the hanging drop version of the
> vapour technique use:
> 
> McPherson, A. (1976) Methods Biochem. Anal. 23, 249-345
> or
> McPherson A. Crystallization of biological macromolecules. Cold Spring
> Harbor, NY: Cold Spring Harbor Laboratory Press; 1999.
> (or McPherson A. Preparation and Analysis of Protein Crystals. New York:
> Wiley, 1982).
> 
> Best
> 
> Jeroen
> 
> Am 17.03.18 um 07:00 schrieb Shane Caldwell:
> > Hi ccp4bb,
> > 
> > I've been asked to provide a reference for making hanging-drop crystals,
> > and I suppose that I never realized the need to cite the technique
> > specifically. Is there a definitive publication where one could trace
> > the origins of the technique, or a particularly thorough textbook or
> > review?
> > 
> > Shane Caldwell
> > shane.caldwel...@gmail.com 
> -- 
> Dr.math. et dis. nat. Jeroen R. Mesters
> Deputy, Senior Researcher & Lecturer
> Program Coordinator /Infection Biology/ 
> 
> 
> Institute of Biochemistry, University of Lübeck
> Ratzeburger Allee 160, 23538 Lübeck, Germany
> phone: +49-451-31013105 (secretariate -31013101)
> fax: +49-451-31013104
> 
> http://jobs.zeit.de/image-upload/logo_10564.jpg
> http://www.biochem.uni-luebeck.de 
> http://www.eine-stadt-sieht-gelb.de 
> http://www.uni-luebeck.de/studium/studiengaenge/infection-biology
> http://www.iobcr.org 
> 
> Visiting Professorship in Biophysics, University of South Bohemia (CZ)
> President of the International Organization for Biological Crystallization
> (IOBCr)
> --
> If you can look into the seeds of time and tell which grain will grow and
> which will not, speak then to me who neither beg nor fear (Shakespeare's
> Macbeth, Act I, Scene 3)
> --
> "Aujourd'hui je sais qu'il n'y a pas de limites à la bêtise humaine -
> qu'elle est infinie." (Gustave Flaubert, French novelist, 1821-1880)
> --
> It is invariably the case that high resolution X-ray structures show
> significantly better agreement with solution observables such as coupling
> constants, 13C chemical shifts, and proton chemical shifts, than the
> corresponding NMR structures, including the very best ones. Hence, in most
> cases, a high-resolution crystal structure (< 2.0 Å)will provide a better
> description of the structure in solution than the corresponding NMR
> structure  (Kuszewski, Gronenborn & Clore, 1996, Protein Science 5:1067-80)
> --
> Disclaimer
> * This message contains confidential information and is inte

[ccp4bb] Wellcome Trust 4-year PhD Interdisciplinary Programme in Structural, Computational and Chemical Biology

[Cara Vaughan]

Dear Colleagues
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