[ccp4bb] Phaser_EP

[Ylva Lindqvist]

After updating CCP4 includin Phaser_EP I can not run it - it can not read my 
SIGFs - what is wrong? The error message is " SYNTAX ERROR: Use SIGFPOS SIGF(+) 
or SIG+ " but my mtz-labels are LABIN F+ = F_Fe1(+) SIGF+ = SIGF_Fe1(+) F- = 
F_Fe1(-) SIGF- = SIGF_Fe1(-). I have changed them with SFTOOLS but that did not 
help - always the same error message.

Ylva Lindqvist

Dept. of Medical Biochemistry & Biophysics
Div. of Molecular Structural Biology
KAROLINSKA INSTITUTET
Tomtebodavägen 6
S-171 77 Stockholm, Sweden

Re: [ccp4bb] Phaser_EP

[Ylva Lindqvist]

Could my problem be because of switching from Windows_XP to Windows7???

Prof. Ylva Lindqvist

Dept. of Medical Biochemistry & Biophysics
Div. of Molecular Structural Biology
KAROLINSKA INSTITUTET
Tomtebodavägen 6
S-171 77 Stockholm, Sweden

Phone   +46 8 52487695
Mobile +46 708 264356
Fax +46 8 327626

Email: ylva.lindqv...@ki.se
URL: http://www.msb.mbb.ki.se/

Re: [ccp4bb] expression of membrane proteins as GST fusions

[Catarina Rodrigues]

Hi,

To purify your protein you can use GST-Trap HP column, it is quite easy, you 
have to elute in a buffer with 20mM reduced glutathione.

HTH,

Catarina
-- 
Catarina Rodrigues, PhD Student
Molecular Transport & Signalling
AFMB UMR 6098 CNRS/UI/UII Case 932 
163 Avenue de Luminy 
13288 Marseille cedex 9 (France) 
Tel : +33 04 91 82 55 60
Fax : 04 91 26 67 20
e-mail:catarina.rodrig...@afmb.univ-mrs.fr
http://www.afmb.univ-mrs.fr/




Le 27 mai 2011 à 22:50, Pascal Egea a écrit :

> 
> Dear All,
> 
> This is not strictly a crystallography related question.
> We are trying to express several membrane proteins and were considering the 
> use of GST as a fusion partner instead of the HIS or FLAG purification tags.
> I would like to know if anyone had any experience (positive or negative) to 
> share with us. 
> 
> Many thanks in advance.
> 
> -- 
> Pascal F. Egea, PhD
> Assistant Professor
> UCLA, David Geffen School of Medicine
> Department of Biological Chemistry
> 364 Boyer Hall
> Molecular Biology Institute
> office (310)-983-3515
> lab  (310)-983-3516
> email pe...@mednet.ucla.edu
> 
> 
> -- 
> Pascal F. Egea, PhD
> Assistant Professor
> UCLA, David Geffen School of Medicine
> Department of Biological Chemistry
> 364 Boyer Hall
> Molecular Biology Institute
> office (310)-983-3515
> lab  (310)-983-3516
> email pe...@mednet.ucla.edu
> 
> 
> -- 
> This message has been scanned for viruses and 
> dangerous content by MailScanner, and is 
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