> On Nov 14, 2017, at 12:49 PM, Fix Ace <ace...@rocketmail.com> wrote:
> 
> Hi, David,
> 
> Thank you very much for getting back to me! Sorry about the messy code 
> example. I am re-posting here (including the error message):
> 
> > example.3=data.frame(levels=as.numeric(XXX[,c(4)]),replicate=rep(c("0","1","2","3","4","5"),3),conditions=c(rep("11",6),rep("12",6),rep("13",6)))
> > example.3
>     levels replicate conditions
> 1  43.1111         0         11
> 2  42.0942         1         11
> 3  57.8131         2         11
> 4  57.1726         3         11
> 5  77.8678         4         11
> 6  44.7578         5         11
> 7  69.5078         0         12
> 8  52.0581         1         12
> 9  40.0602         2         12
> 10 45.5487         3         12
> 11 43.6201         4         12
> 12 60.4939         5         12
> 13 64.1932         0         13
> 14 53.4055         1         13
> 15 59.6701         2         13
> 16 52.6922         3         13
> 17 53.8712         4         13
> 18 60.2770         5         13
> > m.example.3=lmer(as.numeric(levels)~conditions+(conditions|replicate),data=example.3)
> Error: number of observations (=18) <= number of random effects (=18) for 
> term (conditions | replicate); the random-effects parameters and the residual 
> variance (or scale parameter) are probably unidentifiable

The error message seems fairly clear. The formula you have provided is asking 
for estimation of too many parameters. I think you probably want:

m.example.3=lmer(levels~conditions+(1|replicate),data=example.3)

... although your description of the hypothesis under test is ... non-existent.

-- 
David.
> > 
> 
> Please let me know if it is readable this time. 
> 
> Again, many thanks for your time and please help me fix the issue.
> 
> Kind regards,
> 
> Ace
> 
> 
> On Tuesday, November 14, 2017 12:19 PM, David Winsemius 
> <dwinsem...@comcast.net> wrote:
> 
> 
> 
> > On Nov 14, 2017, at 5:13 AM, Fix Ace via R-help <r-help@r-project.org> 
> > wrote:
> > 
> > Dear R Community,
> > My data have 3 conditions and each condition has 6 replicates. I am trying 
> > to fit my data for a linear mixed model using the lmer function from lme4 
> > package to find the random effects of the replicates;
> 
> Better venue for this question might be SIG-mixed-models. See the link 
> avaialble at the bottom of every posting from rhelp:
> 
> https://stat.ethz.ch/mailman/listinfo/r-help:
> 
> 
> 
> 
> > however, I got the error message. Here are the example codes:
> >> example.3=data.frame(levels=as.numeric(XXX[,c(4)]),replicate=rep(c("0","1","2","3","4","5"),3),conditions=c(rep("11",6),rep("12",6),rep("13",6)))>
> >>  example.3    levels replicate conditions1  43.1111        0         112  
> >> 42.0942        1        113  57.8131        2        114  57.1726        3 
> >>        115  77.8678        4        116  44.7578        5        117  
> >> 69.5078        0        128  52.0581        1        129  40.0602        2 
> >>        1210 45.5487        3        1211 43.6201        4        1212 
> >> 60.4939        5        1213 64.1932        0        1314 53.4055        1 
> >>        1315 59.6701        2        1316 52.6922        3        1317 
> >> 53.8712        4        1318 60.2770        5        13> 
> >> m.example.3=lmer(as.numeric(levels)~conditions+(conditions|replicate),data=example.3)Error:
> >>  number of observations (=18) <= number of random effects (=18) for term 
> >> (conditions | replicate); the random-effects parameters and the residual 
> >> variance (or scale parameter) are probably u
 nidentifiable> 
> > Could anyone help me figure out how to fix the issue? 
> > Thank you very much for any inputs!
> > Ace
> 
> > 
> >     [[alternative HTML version deleted]]
> 
> Complete mess. If you haven't yet been advised to posting in plain text, then 
> this should be your wakeup call. If you have, then why are you ignoring 
> sensible advice?
> 
> 
> > 
> > ______________________________________________
> > R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see
> > https://stat.ethz.ch/mailman/listinfo/r-help
> > PLEASE do read the posting guide http://www.R-project.org/posting-guide.html
> > and provide commented, minimal, self-contained, reproducible code.
> 
> David Winsemius
> Alameda, CA, USA
> 
> 'Any technology distinguishable from magic is insufficiently advanced.'  
> -Gehm's Corollary to Clarke's Third Law
> 
> 
> 
> 
> 
> 
> 

David Winsemius
Alameda, CA, USA

'Any technology distinguishable from magic is insufficiently advanced.'   
-Gehm's Corollary to Clarke's Third Law

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