Jose Avalos wrote:
Dear all,
Is it possible to do a movie in which you start looking at the entire
enzyme and then you zoom into the active site and finally alternate
between different states? If so, can somebody please point me in the right
direction?
Thank you very much
Jose Avalos
It certainly is possible, but not using the "mdo" commands in pymol (as
far as I know). (Actually, a simple linear zoom like the first part of
what I describe below might be possible.)
I made a similar movie (the non-proprietary part of which I can send you
if you want) using a script file that looked like this:
(load, color, and render molecules)
# start loop here
#
move z, 5
png movie_1
move x, 1.9
move y, 1.3
move z, 14.7
png movie_2
move x, 1.9
move y, 1.3
move z, 14.7
etc. etc.
move x, 1.805
move y, 1.235
move z, 13.965
turn x, -0.3
turn y, 0.1
png movie_42
move x, 1.71
move y, 1.17
move z, 13.23
turn x, -0.6
turn y, 0.2
png movie_43
move x, 1.615
move y, 1.105
move z, 12.495
turn x, -0.9
turn y, 0.3
etc. etc.
This looks best (most realistic) if rendered in perspective mode, which
means not raytraced. This will also make rendering faster.
The first 40 frames of the zoom are just a linear motion, while the last
40 frames are a steady deceleration of the linear motion, coupled with a
sinusoidal rate of turn in x and y (slow, then faster, then slower) to
bring the view to a gradual halt at the correct position and
orientation. This involved some trial and error, as you might imagine.
I generated the numbers you see above in Excel, then used awk to create
the script file. Setting the origin to the correct location makes sure
the turns are centered around the right point.
Hope that wasn't too intimidating! Good luck - this can be really
impressive when it's finished.
- Matt
--
Matthew Franklin Phone:(650)225-4596
Postdoctoral Researcher Fax:(650)225-3734
Genentech, Inc.
1 DNA Way, South San Francisco, CA 94080
