Thanks for your advice Viktor
I have done a capture and loaded into WireShark.


1,"0.000000","1.2.3.4","192.168.1.239","TCP","66","19524 > smtp [SYN] Seq=0
Win=8192 Len=0 MSS=1380 WS=256 SACK_PERM=1"
2,"0.000022","192.168.1.239","1.2.3.4","TCP","66","smtp > 19524 [SYN, ACK]
Seq=0 Ack=1 Win=5840 Len=0 MSS=1460 SACK_PERM=1 WS=128"
3,"0.043797","1.2.3.4","192.168.1.239","TCP","60","19524 > smtp [ACK] Seq=1
Ack=1 Win=131072 Len=0"
4,"0.047331","192.168.1.239","1.2.3.4","SMTP","96","S: 220 spambox.co.nz
ESMTP Postfix"
5,"0.093672","1.2.3.4","192.168.1.239","SMTP","90","C: EHLO
remote.sender.com"
6,"0.093699","192.168.1.239","1.2.3.4","TCP","54","smtp > 19524 [ACK] Seq=43
Ack=37 Win=5888 Len=0"
7,"0.095070","192.168.1.239","1.2.3.4","SMTP","261","S: 250-spambox.co.nz |
250-PIPELINING | 250-SIZE 20728640 | 250-VRFY | 250-ETRN | 250-STARTTLS |
250-AUTH PLAIN LOGIN CRAM-MD5 | 250-AUTH=PLAIN LOGIN CRAM-MD5 |
250-ENHANCEDSTATUSCODES | 250-8BITMIME | 250 DSN"
8,"0.140698","1.2.3.4","192.168.1.239","SMTP","115","C: MAIL
FROM:<jono.sm...@sender.com> SIZE=221190"
9,"0.146588","192.168.1.239","1.2.3.4","SMTP","68","S: 250 2.1.0 Ok"
10,"0.190450","1.2.3.4","192.168.1.239","SMTP","89","C: RCPT
TO:<recipi...@domain.com>"
11,"0.230190","192.168.1.239","1.2.3.4","TCP","54","smtp > 19524 [ACK]
Seq=264 Ack=133 Win=5888 Len=0"
12,"3.625029","192.168.1.239","1.2.3.4","SMTP","68","S: 250 2.1.5 Ok"
13,"3.668700","1.2.3.4","192.168.1.239","SMTP","60","C: DATA"

Which follows on for a while transmitting then this occurs

66,"4.267143","192.168.1.239","1.2.3.4","TCP","54","smtp > 19524 [ACK]
Seq=315 Ack=47059 Win=64128 Len=0"
67,"4.272844","1.2.3.4","192.168.1.239","SMTP","1434","C: DATA fragment,
1380 bytes"
68,"4.278587","1.2.3.4","192.168.1.239","SMTP","1434","C: DATA fragment,
1380 bytes"
69,"4.278597","192.168.1.239","1.2.3.4","TCP","54","smtp > 19524 [ACK]
Seq=315 Ack=49819 Win=64128 Len=0"
70,"4.284366","1.2.3.4","192.168.1.239","SMTP","1434","C: DATA fragment,
1380 bytes"
71,"4.290093","1.2.3.4","192.168.1.239","SMTP","1434","[TCP Previous segment
not captured] C: DATA fragment, 1380 bytes"
72,"4.290104","192.168.1.239","1.2.3.4","TCP","66","smtp > 19524 [ACK]
Seq=315 Ack=51199 Win=64128 Len=0 SLE=53959 SRE=55339"
73,"4.295853","1.2.3.4","192.168.1.239","SMTP","1434","C: DATA fragment,
1380 bytes"
74,"4.295859","192.168.1.239","1.2.3.4","TCP","66","[TCP Dup ACK 72#1] smtp
> 19524 [ACK] Seq=315 Ack=51199 Win=64128 Len=0 SLE=53959 SRE=56719"
75,"4.301731","1.2.3.4","192.168.1.239","SMTP","1434","C: DATA fragment,
1380 bytes"
76,"4.301738","192.168.1.239","1.2.3.4","TCP","66","[TCP Dup ACK 72#2] smtp
> 19524 [ACK] Seq=315 Ack=51199 Win=64128 Len=0 SLE=53959 SRE=58099"
77,"4.307588","1.2.3.4","192.168.1.239","SMTP","1434","[TCP Previous segment
not captured] C: DATA fragment, 1380 bytes"

















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