Thank you for the response!

Below is the command line I used:

mri_vol2surf --srcsubject Tract_HC_001_07222016 --hemi lh --projopt 
/autofs/space/allegro_002/users/hlee/SANDI/pvf/Tract_HC_001_07222016/pvf 
--projfrac 0 --surf white --srcreg 
/autofs/space/rhapsody_001/users/Bay8_C1/bids/derivatives/supres/sub-001/bbr/diff_bbr_t1w.dat
 --src 
/autofs/cluster/connectome2/Bay8_C1/bids/derivatives/SANDI/supres/sub-001/sub-001-SANDI-fit_De.nii.gz
 --out 
/autofs/cluster/connectome2/Bay8_C1/bids/derivatives/SANDI/supres/surf_pvf/sub-001/lh.De.100.overlay.mgz
 –cortex

I have the following files pvf.gm.mgz, pvf.wm.mgz, pvf.csf.mgz, and 
pvf.subcort_gm.mgz in the directory: 
/autofs/space/allegro_002/users/hlee/SANDI/pvf/Tract_HC_001_07222016.

Please let me know if you need additional details.


Thanks!
Hansol

From: freesurfer-boun...@nmr.mgh.harvard.edu 
<freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Douglas N. Greve 
<dgr...@mgh.harvard.edu>
Date: Tuesday, November 12, 2024 at 10:55 AM
To: freesurfer@nmr.mgh.harvard.edu <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] mri_vol2surf error: GM estimation couldn't find 
enough samples
what is your command line?

On 11/11/2024 3:47 PM, Lee, Hansol wrote:
Dear FreeSurfer developers,


I am attempting to use “mri_vol2surf” command to project output from diffusion 
MRI models (e.g., DTI) onto the cortical surface.

The registration between the diffusion MRI and anatomical space was performed 
using “bbregister”, and the projection works well for different --projfrac 
values (e.g., 0 to 1 from the white matter surface).

However, I encountered issues when attempting to use the “—projopt” option to 
account for partial volume effects. Specifically, I received the following 
errors:

“reading gm volume fraction from 
/autofs/space/allegro_002/users/hlee/SANDI/pvf/Tract_HC_001_07222016/pvf.gm.mgz
reading wm volume fraction from 
/autofs/space/allegro_002/users/hlee/SANDI/pvf/Tract_HC_001_07222016/pvf.wm.mgz
reading csf volume fraction from 
/autofs/space/allegro_002/users/hlee/SANDI/pvf/Tract_HC_001_07222016/pvf.csf.mgz
error: No such file or directory
error: GM estimation at vno 0 couldn't find enough samples
error: No such file or directory
error: GM estimation at vno 1 couldn't find enough samples
error: No such file or directory
error: GM estimation at vno 2 couldn't find enough samples
error: No such file or directory
error: GM estimation at vno 3 couldn't find enough samples
error: No such file or directory
error: GM estimation at vno 4 couldn't find enough samples
error: No such file or directory
error: GM estimation at vno 5 couldn't find enough samples
error: No such file or directory”

In the directory, I already have “pvf.gm.mgz”, “pvf.wm.mgz”, and “pvf.csf.mgz” 
files.

I searched the archive and found a similar error reported in this thread:
https://www.mail-archive.com/freesurfer@nmr.mgh.harvard.edu/msg72784.html

I would greatly appreciate any insights or suggestions to resolve this issue.
Thank you in advance for your help!


Best regards,
Hansol Lee




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