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Dear Yujing,
Thank you for your reply.  Would there be a way to just use the mask created 
with optiBET for the recon-all process to mask the brain? At the moment we are 
segmenting 2 images at once (mprage and mp2rage) to improve the quality of the 
segmentation and we have the skull-stripped images only for one of them so the 
solution below might not be ideal.

Thanks again.

Best,
Irene

From: freesurfer-boun...@nmr.mgh.harvard.edu 
<freesurfer-boun...@nmr.mgh.harvard.edu> On Behalf Of Huang, Yujing
Sent: venerdì 29 dicembre 2023 15:44
To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] recon all with optiBET brain mask

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harvard. edu/fswiki/UserContributions/FAQ Q. I have already skull-stripped 
data. Can

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Q. I have already skull-stripped data. Can I submit it to recon-all?
A: If your skull-stripped volume does not have the cerebellum, then no. If it 
does, then yes, however you will have to run the data a bit differently.
First you must run only -autorecon1 like this:
recon-all -autorecon1 -noskullstrip -s <subjid>
Then you will have to make a symbolic link or copy T1.mgz to brainmask.auto.mgz 
and a link from brainmask.auto.mgz to brainmask.mgz. Finally, open this 
brainmask.mgz file and check that it looks okay (there is no skull, cerebellum 
is intact; use the sample subject bert that comes with your FreeSurfer 
installation to make sure it looks comparable). From there you can run the 
final stages of recon-all:
recon-all -autrecon2 -autorecon3 -s <subjid>
Hope it helps.
 Best,
Yujing


From: 
freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>
 
<freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
 On Behalf Of BALBONI Irene
Sent: Tuesday, December 19, 2023 3:43 AM
To: freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>
Subject: [Freesurfer] recon all with optiBET brain mask


        External Email - Use Caution
Hello,

I would like to run recon all on a batch of subjects using 2 inputs (mprage and 
mp2rage). I would also like to run recon all using as brain mask not the 
standard brain mask generated by recon-all itself but a previously created 
optiBET. Would you have any suggestion on how to proceed? I tried simply 
replacing the brainmask file with the optiBET but it did not work.
Just for context all my participants (150) have been already segmented using 
recon-all but we noticed during quality control that we still have skull 
stripping and pial surface errors for the majority of them and that is why we 
would like to implement the optiBET mask.

Thanks for your help,
Irene Balboni
PhD student
She/Her

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