The vol structure will reflect how the volume is stored on disk. If you have two files that have the same structure (ie, order of rows, cols, slices) then they will have the same vol in matlab regardless of the header orientation.

On 1/23/2024 11:00 AM, Fotiadis, Panagiotis wrote:

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Thank you both! I am asking because I took a nifti file that was in RAS orientation (file1), flipped it into LAS (using /fslorient -swaporient file2/), and then opened both files in MATLAB with MRIread. The resulting .vol matrices for both files were, however, identical (i.e., voxel at location <x,y,z> in file1.vol had the exact same value as voxel in location <x,y,z> in file2.vol). Would you happen to know if the indexing of the vol structure is expected to come out the same with MRIread, regardless of the orientation?

Thanks,

Panos

*From: *freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Iglesias Gonzalez, Juan E. <jiglesiasgonza...@mgh.harvard.edu>
*Date: *Tuesday, January 23, 2024 at 9:14 AM
*To: *Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
*Subject: *Re: [Freesurfer] MRIread and RAS orientation

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I’m pretty sure that the Matlab code does not reorient.

Cheers,

Eugenio

--

Juan Eugenio Iglesias

http://www.jeiglesias.com

*From: *freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Huang, Yujing <yhuan...@mgh.harvard.edu>
*Date: *Tuesday, January 23, 2024 at 08:49
*To: *Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
*Subject: *Re: [Freesurfer] MRIread and RAS orientation

I’m not sure about the MATLAB codes. When the C codes read in images, no automatically transformation is done.

I think you can verify it by checking several [c, r, s] locations of both images loaded with MRIread().  I think c and r are reversed in MATLAB. And the index starts from 1.

Best,

Yujing

*From:*freesurfer-boun...@nmr.mgh.harvard.edu <freesurfer-boun...@nmr.mgh.harvard.edu> *On Behalf Of *Fotiadis, Panagiotis
*Sent:* Tuesday, January 23, 2024 1:54 AM
*To:* freesurfer@nmr.mgh.harvard.edu
*Subject:* [Freesurfer] MRIread and RAS orientation

*        External Email - Use Caution *

Hello,

I have two nifti files that are both in the same MNI space, the former of which is saved in LAS orientation while the other in RAS orientation (as showcased by the “Orientation” field of mri_info). I just wanted to verify: When loading both on MATLAB using MRIread, both of their volumes are automatically loaded (or transformed in the case of the first file) in the same RAS orientation, right (using the vox2ras transformation)?

Thanks in advance!

Best,

Panos

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