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I am attempting to run white matter tractography using TRACULA, as described in the tutorial, however, using a brain parcellation acquired using an external algorithm. I have put the aparc+aseg.mgz file in the subject directory in folder mri but I am getting the following error: ERROR: Option syn/acomm.flt.prep.trk unknown Linux GS63VR-6RF 5.19.0-43-generic #44~22.04.1-Ubuntu SMP PREEMPT_DYNAMIC Mon May 22 13:39:36 UTC 2 x86_64 x86_64 x86_64 GNU/Linux I also get the following error in the start of the prior fitting step in case they are linked: #@# Priors Mon Jun 12 11:43:04 AM BST 2023 error: Could not create temporary file in /tmp $slistfile: Ambiguous. The error occurs when running the prior calculation step using the prep command: trac-all -c config_file -prep. I've searched the list and no similar errors have been reported. Does anyone have any thoughts on how to troubleshoot this one? 1) FreeSurfer version: freesurfer-7.3.2 2) Platform: ubuntu-22.04.02 _______________________________________________ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer The information in this e-mail is intended only for the person to whom it is addressed. If you believe this e-mail was sent to you in error and the e-mail contains patient information, please contact the Mass General Brigham Compliance HelpLine at https://www.massgeneralbrigham.org/complianceline <https://www.massgeneralbrigham.org/complianceline> . Please note that this e-mail is not secure (encrypted). If you do not wish to continue communication over unencrypted e-mail, please notify the sender of this message immediately. Continuing to send or respond to e-mail after receiving this message means you understand and accept this risk and wish to continue to communicate over unencrypted e-mail.