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Dear Doug,
The "template_coreg.mgz" image is also upside-down and rotated 90 degrees (X).  
Can you suggest how I might troubleshoot the initial registration?  

I can send a logfile if it helps (not sure if I attach the textfile it will 
pass through your email filter) 

I saw a section in "Samseg.py" concerning SAMSEG_LEGACY_REGISTRATION but I'm 
not sure if this is relevant.
Sincerely,Randolph Andrews


 # 
=======================================================================================
        #
        # Preprocessing (reading and masking of data)
        #
        # 
=======================================================================================

        # Read the image data from disk and crop

        # Historically, the template was resaved with a transformed header and 
the image-to-image transform
        # was later extracted by comparing the input image and coregistered 
template transforms, but shear
        # cannot be saved through an ITK image, so a better method is to pass 
the image-to-image transform matrix
        # directly to samseg. If the SAMSEG_LEGACY_REGISTRATION env var is set, 
this old method is enabled
        if os.environ.get('SAMSEG_LEGACY_REGISTRATION') is not None:
            print('INFO: using legacy (broken) registration option')
            transformedTemplateFileName = os.path.join(self.savePath, 
'template_coregistered_legacy.nii')
            self.imageBuffers, self.transform, self.voxelSpacing, self.cropping 
= readCroppedImagesLegacy(self.imageFileNames, transformedTemplateFileName)
        else:
            self.imageBuffers, self.transform, self.voxelSpacing, self.cropping 
= readCroppedImages(
                self.imageFileNames,
                os.path.join(self.atlasDir, 'template.nii'),
                self.imageToImageTransformMatrix
            )


 
-----Original Message-----
From: Douglas N. Greve <dgr...@mgh.harvard.edu>
To: freesurfer@nmr.mgh.harvard.edu
Sent: Sun, Oct 3, 2021 8:04 am
Subject: Re: [Freesurfer] SAMSEG output orientation not the same as input images

 Can you compare the template_coreg.mgz to the input image; they should be 
mostly aligned. If not, then the initial registration failed.
 
 
 On 9/28/2021 2:38 PM, daedalu...@aol.com wrote:
  
         External Email - Use Caution          We have recently installed 
FS-7.1 and have tested a few “ADNI” images with SAMSEG.  The process runs to 
completion, but the output (seg.mgz) appears upside-down and rotated about 90 
about the X-axis.  We are passing seg.mgz through BrainStorm to create NiFTI 
images.  We do not believe that this is a BrainStorm issue inasmuch as the same 
images run through recon-all (fully separate proc) yield output images in the 
same orientation as the original images.  I found a thread from 2012 with a 
similar output orientation issue, but the I did not see a response that 
resolved this. 
  Any solution or insight would be greatly appreciated 
  Sincerely, Randolph Andrews 
   
  (PS. listserv item from 2012) Re: [Freesurfer] integrating FSL and Freesurfer 
Chris Filo Gorgolewski Tue, 09 Oct 2012 01:03:57 -0700 
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