Dear Experts,

I have functional ROIs on the left and right hemispheres, and I’m trying to do 
a bilateral seed to whole brain connectivity analysis.
My question is: in order to make a bilateral seed, shall I manually average the 
.dat files (output of fcseed-sess) from left hemi and right hemi to make a 
bilateral seed (see below)?

fcseed-config  -segid 1 -seg lh.mgz  -fcname lh.dat -fsd rest -rlf restruns 
-mean -cfg lh.config
fcseed-sess -s . -d . -cfg lh.config
fcseed-config  -segid 1 -seg rh.mgz  -fcname rh.dat -fsd rest -rlf restruns 
-mean -cfg rh.config
fcseed-sess -s . -d . -cfg rh.config

bilateral.dat= (lh.dat+rh.dat)/2

OR

should I use the left and right ROIs in fcseed-config at the same time, so the 
out put of fcseed-sess be the bilateral seed? Please see below commands:

fcseed-config  -segid 1 -seg lh.mgz -segid 1 -seg rh.mgz -fcname bi.dat -fsd 
rest -rlf restruns -mean -cfg bi.config
fcseed-sess -s . -d . -cfg bi.config

I appreciate your responses.

Thanks,
Mona



Zahra (Mona) Nasiriavanaki, M.D.
Postdoctoral Research Fellow
Department of Psychiatry | MGH/HMS
149 13th Street, 149-2615
Boston, MA, USA, 02129

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