You can use mri_binarize to output nii.gz files. FSL may or may not read 
them properly as they are surface overlays, not volume. When we convert 
a surface overlay to nifti, we try to repackage the dimensions to fit in 
the nifit standard. Depending on the subject, it sometimes works and 
sometimes does not.

On 6/15/2020 9:53 AM, Mason Wells wrote:
>          External Email - Use Caution
>
> Hi Tim,
>
> My knowledge on file format isn’t brilliant. What I would like to do is 
> compare the spatial overlap between ROIs, which I have been doing in the 
> volume using fslcc. This runs cross correlations between two NIFTI files. 
> However, I wondered if Freesurfer have the facility to do these comparisons 
> on the surface. For example, cross correlating an ICA component and a 
> probabilistic atlas to check the spatial overlap. How I thought I would do 
> this is to get each atlas ROI into different label files, and then use some 
> form of freesurfer command to cross correlate the ICA component to each of 
> the atlas regions and check which is the best "match".
>
> Any tips on how to do this would be appreciated.
>
> Thanks,
> Mason
>
> Mason T Wells, MSc
> PhD student
> School of Optometry and Vision Sciences
> & Cardiff University Brain Research
> Imaging Centre (CUBRIC), School of Psychology
> Cardiff University
> Cardiff
> CF24 4HQ
> UK
>   
> Email: wells...@cardiff.ac.uk
> Tel: 02920 879628
> Web: Cardiff University webpage 
> <https://www.cardiff.ac.uk/people/research-students/view/974214->
> Mason T Wells, MSc
> Myfyriwr PhD
> Yr Ysgol Optometreg a Gwyddorau’r Golwg
> & Canolfan Ymchwil Delweddu’r Ymennydd Prifysgol Caerdydd (CUBRIC), Yr Ysgol 
> Seicoleg
> Prifysgol Caerdydd
> Heol Maindy
> Caerdydd
> CF24 4HQ
> DU
>   
> E-bost: wells...@caerdydd.ac.uk
> Ffôn: 02920 879628
>   
>
> On 15/06/2020, 12:19, "freesurfer-boun...@nmr.mgh.harvard.edu on behalf of 
> Tim Schäfer" <freesurfer-boun...@nmr.mgh.harvard.edu on behalf of 
> ts...@rcmd.org> wrote:
>
>              External Email - Use Caution
>
>      What would you expect to be in that NIFTI file?
>
>      A vector of integers of length the number of surface vertices, in which 
> a value of 0 means "is not part of label" and a value of 1 means "is part of 
> label"?
>
>      Or just the indices of the vertices which are part of the label?
>
>      What software should be able to read the file?
>
>      Tim
>
>      > On June 15, 2020 at 12:15 PM Mason Wells <wells...@cardiff.ac.uk> 
> wrote:
>      >
>      >
>      >         External Email - Use Caution
>      >
>      > Hi Bruce and Doug,
>      >
>      > I tried to load the overlay into Matlab, but that doesn’t work, I just 
> get a series of letters/symbols. The surface overlay is in .mgz format. Using 
>  mri_cor2label works great for getting each roi into a label format, but I 
> would also like them each in a .nii.gz format. Do you have any suggestions 
> for this?
>      >
>      > Cheers,
>      > Mason
>      >
>      > Mason T Wells, MSc
>      > PhD student
>      > School of Optometry and Vision Sciences
>      > & Cardiff University Brain Research
>      > Imaging Centre (CUBRIC), School of Psychology
>      > Cardiff University
>      > Cardiff
>      > CF24 4HQ
>      > UK
>      >
>      > Email: wells...@cardiff.ac.uk<mailto:wells...@cardiff.ac.uk>
>      > Tel: 02920 879628
>      > Web: Cardiff University 
> webpage<https://www.cardiff.ac.uk/people/research-students/view/974214->
>      > Mason T Wells, MSc
>      > Myfyriwr PhD
>      > Yr Ysgol Optometreg a Gwyddorau’r Golwg
>      > & Canolfan Ymchwil Delweddu’r Ymennydd Prifysgol Caerdydd (CUBRIC), Yr 
> Ysgol Seicoleg
>      > Prifysgol Caerdydd
>      > Heol Maindy
>      > Caerdydd
>      > CF24 4HQ
>      > DU
>      >
>      > E-bost: wells...@caerdydd.ac.uk<mailto:wells...@caerdydd.ac.uk>
>      > Ffôn: 02920 879628
>      >
>      >
>      > From: <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of "Fischl, 
> Bruce" <bfis...@mgh.harvard.edu>
>      > Reply to: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
>      > Date: Saturday, 13 June 2020 at 03:02
>      > To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
>      > Subject: Re: [Freesurfer] Splitting surface overlay into separate 
> files {Disarmed}
>      >
>      > Hi Mason
>      >
>      > You should be able to load the overlay and the ROIs into matlab, then 
> pick out the vertices in each ROI from the overlay and save them separately. 
> Just be careful about the one-based indexing in matlab. That is, if your ROI 
> is in a label file and you read the vertex indices from it, you need to add 1 
> to the index that point into the overlay.
>      >
>      > Cheers
>      > Bruce
>      >
>      > From: freesurfer-boun...@nmr.mgh.harvard.edu 
> <freesurfer-boun...@nmr.mgh.harvard.edu> On Behalf Of Mason Wells
>      > Sent: Friday, June 12, 2020 7:35 PM
>      > To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
>      > Subject: Re: [Freesurfer] Splitting surface overlay into separate 
> files {Disarmed}
>      >
>      >
>      >         External Email - Use Caution
>      > Hi Doug,
>      >
>      > Yes, I mean a surface overlay, it is the mgz format. It essentially 
> has 25 visual areas, shown in the attached. I wanted to split them up, so 
> that each ROI is its own label. I saw I could use mri_cor2label, but is there 
> a better way?
>      >
>      > Cheers,
>      > Mason
>      >
>      > Mason T Wells, MSc
>      > PhD student
>      > School of Optometry and Vision Sciences
>      > & Cardiff University Brain Research
>      > Imaging Centre (CUBRIC), School of Psychology
>      > Cardiff University
>      > Cardiff
>      > CF24 4HQ
>      > UK
>      >
>      > Email: wells...@cardiff.ac.uk<mailto:wells...@cardiff.ac.uk>
>      > Tel: 02920 879628
>      > Web: Cardiff University 
> webpage<https://www.cardiff.ac.uk/people/research-students/view/974214->
>      > Mason T Wells, MSc
>      > Myfyriwr PhD
>      > Yr Ysgol Optometreg a Gwyddorau’r Golwg
>      > & Canolfan Ymchwil Delweddu’r Ymennydd Prifysgol Caerdydd (CUBRIC), Yr 
> Ysgol Seicoleg
>      > Prifysgol Caerdydd
>      > Heol Maindy
>      > Caerdydd
>      > CF24 4HQ
>      > DU
>      >
>      > E-bost: wells...@caerdydd.ac.uk<mailto:wells...@caerdydd.ac.uk>
>      > Ffôn: 02920 879628
>      >
>      >
>      > From: 
> <freesurfer-boun...@nmr.mgh.harvard.edu<mailto:freesurfer-boun...@nmr.mgh.harvard.edu>>
>  on behalf of "Douglas N. Greve" 
> <dgr...@mgh.harvard.edu<mailto:dgr...@mgh.harvard.edu>>
>      > Reply to: Freesurfer support list 
> <freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
>      > Date: Friday, 12 June 2020 at 15:44
>      > To: 
> "freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>" 
> <freesurfer@nmr.mgh.harvard.edu<mailto:freesurfer@nmr.mgh.harvard.edu>>
>      > Subject: Re: [Freesurfer] Splitting surface overlay into separate files
>      >
>      > When you say "surface" do you mean a surface overlay or a surface with 
> xyz and neighbor relations? Assuming you mean an overlay (eg, something in an 
> mgh or mgz file), you can try fscalc. Or do you have 25 frames  in a single 
> file and you want to split them into separate files? You might be able to 
> convert the mgh/mgz file to nii and use FSL tools and then convert them back 
> (sometimes works, sometimes not)
>      > On 6/12/2020 10:24 AM, Mason Wells wrote:
>      >
>      >         External Email - Use Caution
>      > Hi Experts,
>      >
>      > Apologies if this is a rather simple question. Is there a FreeSurfer 
> equivalent of fslmaths? I have an atlas overlay that has 25 visual areas in a 
> single .mgz file. I want to separate each atlas region into a different file, 
> so that I end up with 25 surface atlas regions. Is there a command that could 
> achieve this?
>      >
>      > My next question is whether or not there is a Freesurface command that 
> can be used to complete surface based correlations. For example, if I want to 
> check for the overlap between two ROIs. I have been using fslcc to do this in 
> the volume, but perhaps freesurfer has something similar to do this on the 
> surface?
>      >
>      > Cheers,
>      > Mason
>      >
>      > Mason T Wells, MSc
>      > PhD student
>      > School of Optometry and Vision Sciences
>      > & Cardiff University Brain Research
>      > Imaging Centre (CUBRIC), School of Psychology
>      > Cardiff University
>      > Cardiff
>      > CF24 4HQ
>      > UK
>      >
>      > Email: wells...@cardiff.ac.uk<mailto:wells...@cardiff.ac.uk>
>      > Tel: 02920 879628
>      > Web: Cardiff University 
> webpage<https://www.cardiff.ac.uk/people/research-students/view/974214->
>      > Mason T Wells, MSc
>      > Myfyriwr PhD
>      > Yr Ysgol Optometreg a Gwyddorau’r Golwg
>      > & Canolfan Ymchwil Delweddu’r Ymennydd Prifysgol Caerdydd (CUBRIC), Yr 
> Ysgol Seicoleg
>      > Prifysgol Caerdydd
>      > Heol Maindy
>      > Caerdydd
>      > CF24 4HQ
>      > DU
>      >
>      > E-bost: wells...@caerdydd.ac.uk<mailto:wells...@caerdydd.ac.uk>
>      > Ffôn: 02920 879628
>      >
>      >
>      >
>      > _______________________________________________
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