It should give consistent output when re-running. The first thing to 
check is that the version of FS has not changed. In general, you should 
NOT be using the development version. The second thing is to make sure 
that it is the FS output that is changing and not that something has 
changed in your 3rd party scripts.

On 11/8/19 11:16 AM, Chen, Christine wrote:
>
>         External Email - Use Caution
>
> Hello,
>
> My lab has been running T1 (MPRAGE) data through run_subject (uses 
> recon-all, autorecon, mris_volume and mris_wm_volume) then through 
> run.r (pulls freesurfer.r and pedbirn.r) using the Desikan-Killiany 
> atlas to output measures of cortical morphology. We’ve always gotten 
> consistent output when rerunning data, however, lately when rerunning 
> data using the exact same files as before, the output measures are 
> different, sometimes significantly so, compared to when they were 
> previously run.
>
> We recently installed the development version of FreeSurfer in a path 
> different from $FREESURFER_HOME, which is still set to 
> usr/local/freesurfer. We’ve also confirmed that all these runs have 
> been using FreeSurfer v6.0 by looking at the /scripts/build-stamp.txt 
> file. So the inconsistency in output might not be due to the 
> FreeSurfer version used.
>
> Additionally, we are collecting a new dicom format, however when 
> rerunning files collected in the previous dicom format through the 
> pipeline and comparing this output to the output from when it was 
> previously ran, the measures are still different, so the inconsistency 
> in results might not be due to the dicom format.
>
> Do you have any ideas why this sudden change in outputs is being 
> observed? Is there a flag we can add to the scripts to ensure results 
> are identical and consistent with what they were previously, such as 
> –norandomness or –seed 1234?
>
> Any help or guidance would be greatly appreciated. Thank you in advance.
>
> Best,
>
> CC
>
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