that's good to hear, thanks for letting us know Michelle
Bruce
On Mon, 22 Jan 2018, Michelle VanTieghem wrote:

Hi, 
I went ahead and re-processed all of our data with the newest version (6.0)
of freesurfer (I didn't hear back from anyone on this issue for over a
month). It does make a significant difference for our dataset. Pial mater is
no longer labeled as gray matter with version 6, which was a significant
issue for the majority of our images when using  version 5.3. I've attached
screen shot examples her (v5.3 on right, v6 on left). I thought this would
be useful for other users to know! 

Thanks,
Michelle 

On Tue, Jan 2, 2018 at 9:36 AM, Bruce Fischl <fis...@nmr.mgh.harvard.edu>
wrote:
      Hi Michelle

      if you point me at a dataset (an entire subject dir gzipped and
      uploaded to our ftp site) and some voxel coordinates of where
      you see problems, I'll take a look.

      cheers
      Bruce


      On Fri, 22 Dec 2017, Michelle VanTieghem wrote:


            Hi Douglas, 

            I have reported many problems with the skull
            stripping and resulting issues in freesurfer. to
            summarize:

            1. For our structural scans, we have significant
            pial matter/dura matter remaining for about 50% of
            subjects or more (of a dataset of 350 scans). I 
            have tested using freesurfer's gcut and other
            options, as well as AFNI skull strip, FSL with many
            options, and even ANTS. 

            2. We gave up on trying to use surfaces with this
            dataset, due to the sheer amount of manual
            intervention that would be needed to edit the brains

            3. The aseg almost always labels the remaining skull
            as gray matter, which then influences the icv
            and wbv measurements. W

            4. Recently we have decided to try the longitudinal
            pipeline. Another problem appears: if a
            cross-sectional scan does not have any pial matter
            (as in, it is one of the few that as successfully
            skull stripped -  we call these the "clean" scans)
            but a template is made from multiple scans from
            this subject. If one of those other tp scans from
            this subject has any pial mater remaining, then
            the (a) the template will include all of the pial
            matter and (b) the longitudinal version of the
            "clean" scan now has extra pial matter that gets
            erroneously labeled as gray matter, or sometimes
            white matter. Attached is a screenshot example of
            this problem.

            I have been trying to solve this for over a year,
            and I also sent data to freesurfer over the ftp on
            December 1st using the directions on the freesurfer
            wiki, if you want to take a look at a single
            representative scan with this skull strip issue.

            Thanks!

            Michelle 


            ----

            Douglas N Greve Tue, 19 Dec 2017 15:25:14 -0800

            Hi Michelle, sorry about not getting to this before.
            It looks like Bruce was helping you out, but he's
            just left and won't be back until the new year. To
            answer your first question, I don't think that v6
            performs much differently on skull stripping than
            v5.3. What is the nature of the skull stripping
            problem? Can you send screen shots?

            doug


            On Tue, Dec 19, 2017 at 3:47 PM, Michelle VanTieghem
            <michelle.vantieg...@gmail.com> wrote:
                  Hi, 
            I am following up again --  on my prior posts
            regarding skull strip issues and whether
            using freesurfer 6.0 would improve
            over freesurfer 5.3 . 
            I sent the data over the ftp on  Dec 1 and would
            greatly appreciate it if you could take a
            look to see if there is a way to improve the skull
            strip since we have this issue with 100+
            scans and my attempts to use gcut, and other
            skullstrip programs, has not worked.

            Thank you,
            Michelle 

            On Fri, Dec 8, 2017 at 12:31 PM, Michelle VanTieghem
            <michelle.vantieg...@gmail.com> wrote:
                  Hi, 
            I am just following up on my prior posts regarding
            skull strip issues and whether using
            freesurfer 6.0 would improve over freesurfer 5.3 . 
            I sent the data over the ftp and would greatly
            appreciate it if you could take a look to
            see if there is a way to improve the skull strip
            since we have this issue with 100+
            scans and my attempts to use gcut, and other
            skullstrip programs, has not worked.

            Thank you,
            Michelle 

            On Fri, Dec 1, 2017 at 5:08 PM, Michelle VanTieghem
            <michelle.vantieg...@gmail.com>
            wrote:
                  Ok that worked. I sent the data for a subject
            with the skull stripping issue
                  that is common in most of our dataset.
            Thanks,
            Michelle

            On Fri, Dec 1, 2017 at 4:56 PM, Bruce Fischl
            <fis...@nmr.mgh.harvard.edu> wrote:
                  try cd'ing to incoming not outgoing (the names
            are from our
                  perspective I think :)

                  On Fri, 1 Dec 2017, Michelle VanTieghem wrote:


                        yn-128-59-230-36:structural
            michellevantieghem2$ ftp
                        surfer.nmr.mgh.harvard.edu

                        Connected to surfer.nmr.mgh.harvard.edu.

                        220-This is the Martinos Center
            Anonymous FTP service. Use
                        of this site             

                        220-shows implicit consent to our
            Acceptable Use Policy
                        outlined at               

                       
            
220-http://www.nmr.mgh.harvard.edu/martinos/userInfo/computer/nmr-aup.php 
                                 

                        220-

                        220-OPERATIONAL NOTE:  If, when doing a
            GET or PUT, you
                        encounter either 

                        220-

                        220-229 Entering Extended Passive Mode

                        220-

                        220-                   and nothing
            happens OR 

                        220-

                        220-229 Entering Extended Passive Mode

                        220-500 Bad EPRT protocol. 

                        220-

                        220-                  try issuing the
            EPSV command prior
                        to GET or PUT.

                        220-

                        220 

                        Name
            (surfer.nmr.mgh.harvard.edu:michellevantieghem2):
                        anonymous

                        331 Please specify the password.

                        Password: 

                        230 Login successful.

                        Remote system type is UNIX.

                        Using binary mode to transfer files.

                        ftp> cd transfer/outgoing

                        250 Directory successfully changed.

                        ftp> put SB152_freesurfer.tar.gz 

                        local: SB152_freesurfer.tar.gz remote:
                        SB152_freesurfer.tar.gz

                        229 Entering Extended Passive Mode
            (|||37860|).

                        553 Could not create file.

                        ftp> ls

                        229 Entering Extended Passive Mode
            (|||37896|).

                        150 Here comes the directory listing.

                        -rw-r--r--    1 0        0           
            3812 Dec 01 06:10
                        README.1st

                        drwxrwsr-t    4 0        1046        
            4096 Nov 21  2014
                        flat

                        drwxrwsr-t   13 0        160         
            4096 Aug 19  2013
                        fsdev

                        drwxrwsr-t   14 0        1046        
            4096 Dec 05  2011
                        recon

                        drwxrwsr-t    3 0        18          
            4096 Jan 24  2005
                        sysadm

                        226 Directory send OK.

                        ftp> ls

                        229 Entering Extended Passive Mode
            (|||37892|).

                        150 Here comes the directory listing.

                        -rw-r--r--    1 0        0           
            3812 Dec 01 06:10
                        README.1st

                        drwxrwsr-t    4 0        1046        
            4096 Nov 21  2014
                        flat

                        drwxrwsr-t   13 0        160         
            4096 Aug 19  2013
                        fsdev

                        drwxrwsr-t   14 0        1046        
            4096 Dec 05  2011
                        recon

                        drwxrwsr-t    3 0        18          
            4096 Jan 24  2005
                        sysadm

                        226 Directory send OK.

                        ftp> put SB152_freesurfer.tar.gz

                        local: SB152_freesurfer.tar.gz remote:
                        SB152_freesurfer.tar.gz

                        229 Entering Extended Passive Mode
            (|||37898|).

                        553 Could not create file.

                        ftp> 


                        On Fri, Dec 1, 2017 at 4:50 PM, Bruce
            Fischl
                        <fis...@nmr.mgh.harvard.edu> wrote:
                              can you paste the full session
            text into the email
                        (not as a screen shot)?

                              On Fri, 1 Dec 2017, Michelle
            VanTieghem wrote:

                                    Hi, I am unsure whether this
            worked: see
                        attached screenshot.

                                    Thanks!
                                    M

                                    On Fri, Dec 1, 2017 at 4:26
            PM, Bruce Fischl
                        <fis...@nmr.mgh.harvard.edu> wrote:
                                          yes, it should. You
            should just be able
                        to do a "put"
                                          On Fri, 1 Dec 2017,
            Michelle VanTieghem
                        wrote:

                                                Hi, 
                                                I am confused by
            the instructions
                        for the ftp. Once I am logged into the
            ftp and have
                                    navigated to
                                                the
            transfer/incoming
                                                directory, how
            am I supposed to
                        find / access the file that I would ike
            to send
                                    through? Does
                                                terminal still
            recognize my
                                                working
            directory before I logged
                        into the ftp?

                                                Thanks!
                                                Michelle 

                                                On Sat, Nov 18,
            2017 at 2:54 PM,
                        Bruce Fischl
            <fis...@nmr.mgh.harvard.edu> wrote:
                                                      Hi
            Michelle

                                                      probably
            best to tar and
                        gzip the entire subject directory and
            ftp it to us:

                                                     
                       
            https://surfer.nmr.mgh.harvard.edu/fswiki/FtpFileExchange

                                                      cheers
                                                      Bruce

                                                      On Sat, 18
            Nov 2017,
                        Michelle VanTieghem wrote:

                                                            Hi, 
                                                            Yes
            I can send you the
                        data - should I just attach the mprage
            file in the
                                    email, or is
                                                there
                                                           
            another
                                                            way
            to share the data
                        with freesurfer? Also, should I send one
            scan or
                                    multiple
                                                scans? 

                                                           
            Thanks,
                                                           
            Michelle

                                                            On
            Sat, Nov 18, 2017
                        at 10:37 AM, Bruce Fischl
                                    <fis...@nmr.mgh.harvard.edu>
            wrote:
                                                               
              Hi Michelle

                                                               
              are you doing
                        anything out of the ordinary? Do you
            want to send us a
                                    dataset and
                                                we will
                                                               
              take a look? I
                        don't think 6.0 is very different from
            5.3 in regard
                                    to skull
                                                stripping,
                                                               
              although it is
                        probably a bit better

                                                               
              Bruce

                                                               
              On Fri, 17 Nov
                        2017, Michelle VanTieghem wrote:

                                                               
                    Hello, 
                                                               
                    I have
                        posted several times about problems with
                                    skull-stripping with our
                                                               
                    dataset.
                        We have tried gcut
                                                               
                    and
                        multiple other options in other packages
            (AFNI, FSL, ANTS)
                                    to remove
                                                               
                    skull from
                        structural
                                                               
                    images in
                        our dataset with little success.

                                                               
                    Recently,
                        a colleague suggested this may be due to
            versions:
                                    that 6.0
                                                deals
                                                               
                    with skull
                        stripping
                                                               
                    much
                        better than 5.3. 

                                                               
                     I wanted
                        to ask if indeed this could be the case
            before I go
                                    install 6.0
                                                               
                    and re-run
                        everything in
                                                               
                    my study.

                                                               
                    Thank
                        you, 
                                                               
                    Michelle 

                                                               
                    --
                                                               
                    Michelle
                        VanTieghem
                                                               
                    PhD
                        student in Psychology
                                                               
                   
                        Developmental Affective Neuroscience Lab
                                                               
                    Columbia
                        University 
                                                               
                   
                        mrv2...@columbia.edu




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                        this e-mail is intended only for the
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                                    is
                                                           
            addressed. If you
                        believe this e-mail was sent to you in
            error and the
                                    e-mail
                                                           
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                        information, please contact the Partners
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                                    HelpLine at
                                                           
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            If the e-mail was
                        sent to you in
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                                    properly
                                                           
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                                                            --
                                                           
            Michelle VanTieghem
                                                            PhD
            student in
                        Psychology
                                                           
            Developmental
                        Affective Neuroscience Lab
                                                           
            Columbia University 
                                                           
            mrv2...@columbia.edu




                                                --
                                                Michelle
            VanTieghem
                                                PhD student in
            Psychology
                                                Developmental
            Affective
                        Neuroscience Lab
                                                Columbia
            University 
                                               
            mrv2...@columbia.edu




                                    --
                                    Michelle VanTieghem
                                    PhD student in Psychology
                                    Developmental Affective
            Neuroscience Lab
                                    Columbia University 
                                    mrv2...@columbia.edu




                        --
                        Michelle VanTieghem
                        PhD student in Psychology
                        Developmental Affective Neuroscience Lab
                        Columbia University 
                        mrv2...@columbia.edu




            --
            Michelle VanTieghem
            PhD student in Psychology
            Developmental Affective Neuroscience Lab
            Columbia University 
            mrv2...@columbia.edu




            --
            Michelle VanTieghem
            PhD student in Psychology
            Developmental Affective Neuroscience Lab
            Columbia University 
            mrv2...@columbia.edu




            --
            Michelle VanTieghem
            PhD student in Psychology
            Developmental Affective Neuroscience Lab
            Columbia University 
            mrv2...@columbia.edu




            --
            Michelle VanTieghem
            PhD student in Psychology
            Developmental Affective Neuroscience Lab
            Columbia University 
            mrv2...@columbia.edu




--
Michelle VanTieghem
PhD student in Psychology
Developmental Affective Neuroscience Lab
Columbia University 
mrv2...@columbia.edu

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