OK, got it.
The problem is that your images are aligned in physical coordinates, but not in 
voxel coordinates. FreeView handles that nicely, but I don’t know about 
FSLview. The subfield code also generates files named *.FSvoxelSpace.mgz, which 
are in the same voxel coordinates as the recon-all files. You can try using 
those instead.
Regarding the voxel size of your T1s: the volumes are always in cubic 
millimeters ;-)
Cheers,
/Eugenio



--
Juan Eugenio Iglesias
ERC Senior Research Fellow
Translational Imaging Group
University College London
http://www.jeiglesias.com
http://cmictig.cs.ucl.ac.uk/


From: <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Erik O'Hanlon 
<erikohan...@rcsi.ie>
Reply-To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Date: Friday, 12 January 2018 at 15:13
To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Subject: Re: [Freesurfer] making masks with subfields etc

Hello Eugenio,

Thanks for your reply. When I'm in freeview, the hippocampus and even the 
amgydala from the aseg align nicely with my input T1 image. I suspect that it 
could be an issue when export ing them from freeview. I just saved the 
hippocampus etc as nii volumes and then tried to open them in FSL view with my 
T1 which has my DWI image aligned but it complains about image size and 
dimensions etc which made me think that the hippocampus volumes etc weren't in 
the same space as my T1. So my files in the subject/mri dir (T1, aseg and hippo 
etc) are all in native space (the same space that my initial T1 image fed into 
reconall is in)?

Sorry for the confusion on my part.

Also as an aside, teh hippocampal subfield measures in the txt files giving the 
volume for each subfield, are they in mm3 or if my T1 is 0.89mm3 do I need to 
adjust for that.

Thanks again for any help.

Best regards

Erik
________________________________
From: freesurfer-boun...@nmr.mgh.harvard.edu 
[freesurfer-boun...@nmr.mgh.harvard.edu] on behalf of Iglesias Gonzalez, 
Eugenio [e.igles...@ucl.ac.uk]
Sent: 12 January 2018 14:21
To: Freesurfer support list
Subject: Re: [Freesurfer] making masks with subfields etc
Dear Erik,
I might be missing something here, but: the subfield segmentation, rawavg.mgz, 
etc, are all already in native subject space…
Cheers,
/E

--
Juan Eugenio Iglesias
ERC Senior Research Fellow
Translational Imaging Group
University College London
http://www.jeiglesias.com
http://cmictig.cs.ucl.ac.uk/


From: <freesurfer-boun...@nmr.mgh.harvard.edu> on behalf of Erik O'Hanlon 
<erikohan...@rcsi.ie>
Reply-To: Freesurfer support list <freesurfer@nmr.mgh.harvard.edu>
Date: Friday, 12 January 2018 at 13:07
To: "freesurfer@nmr.mgh.harvard.edu" <freesurfer@nmr.mgh.harvard.edu>
Subject: [Freesurfer] making masks with subfields etc

Hi FS experts,

I have run a hippocampal analysis with v6.0 including the subfields and am now 
in the process of running an  addtional analysis using the dev6.0 version for 
the amygdala and posterior/anterior hippocampal measures. I also want to use 
the 3D rendered volumes of the hippocampus and amygdala as volume ROI masks for 
tractography.
1. My diffusion data is registered to my T1 data. Just wondering is it the 
rawavg.mgz file I need to register the ROI's to?
2. I'm using freeview and 3D rendererd the Hippocampus (203-226) and saved that 
as a volume. Do I use the mri_vol2vol command to align my roi's to the rawavg 
image for correctly map onto my T1 native space image because when I try that 
it seems that the ROI volume image can't be opened. I think I must be missing a 
step when creating the mask perhaps?
3. If I also wish to generate additional masks from the aseg.mgz, will that be 
done via the same steps used for the hippocampus or do I need to use the 
fslmaths <input imgae> -thr LUT number of the subcorical structure -uthr lut 
number again <output filename> first and then regsiter them to the rawavg.mzg?

Thanks for any guidance which is always greatly appreciated.



Best regards

Erik



Erik O'Hanlon
Postdoctoral researcher

[cid:image001.png@01D38BBB.D10AAF80]

RCSI Psychiatry
Royal College of Surgeons in Ireland
Beaumont Road, Beaumont, D9, Ireland
T: 8093740
E: erikohan...@rcsi.ie W: www.rcsi.ie<http://www.rcsi.ie/>

RCSI DEVELOPING HEALTHCARE LEADERS
WHO MAKE A DIFFERENCE WORLDWIDE


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