Hello. We are trying to use TRACULA to analyze the structural connectivity of 
PD patients scanned on a 3 tesla Philips Ingenia. We have run the tutorial data 
without any problems but encounter errors when using our own data. We ran 
recon-all on all of our subjects without any problem and follow the exact steps 
that worked for the tutorial data but encounter different errors when using the 
dcm or nii version of the DTI scans. Our nii files go from scanner to CD to 
Osirix to DICOM to dcm2nii which gives us the nii files and the bvals and 
bvecs.  Here is the error for the nii DTI scans (included bvals and gradients 
in the config file for the nii files).

[Christinas-iMac:Freesurfer_temp/PD800/diffusion_recons] christina% trac-all 
-prep -c/Volumes/CFERRIER/Freesurfer_temp/PD800/dmrirc.tutorial

ERROR: flag -c/Volumes/CFERRIER/Freesurfer_temp/PD800/dmrirc.tutorial 
unrecognized

-prep -c/Volumes/CFERRIER/Freesurfer_temp/PD800/dmrirc.tutorial

[Christinas-iMac:Freesurfer_temp/PD800/diffusion_recons] christina% trac-all 
-prep -c /Volumes/CFERRIER/Freesurfer_temp/PD800/dmrirc.tutorial

INFO: SUBJECTS_DIR is /Volumes/CFERRIER/Freesurfer_temp/PD800/diffusion_recons

INFO: Diffusion root is /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT

Actual FREESURFER_HOME /Applications/Freesurfer

trac-preproc -c 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/dmrirc.local 
-log 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/trac-all.log 
-cmd 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/trac-all.cmd

#-------------------------------------

/Applications/Freesurfer/bin/trac-preproc

#-------------------------------------

#@# Image corrections Thu Aug 31 14:45:46 CDT 2017

mri_convert --bvec-voxel 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI_combined.nii 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz

mri_convert.bin --bvec-voxel 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI_combined.nii 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz

$Id: mri_convert.c,v 1.226 2016/02/26 16:15:24 mreuter Exp $

reading from 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI_combined.nii...

TR=0.00, TE=0.00, TI=0.00, flip angle=0.00

i_ras = (-0.999339, -0.0363145, -0.00163044)

j_ras = (-0.0363101, 0.99508, 0.0921851)

k_ras = (0.00172524, -0.0921834, 0.995741)

writing to 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz...

cp /Volumes/CFERRIER/Freesurfer_temp/PD800/bvecs/PD800_bvecs.txt 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvecs

cp /Volumes/CFERRIER/Freesurfer_temp/PD800/bvals/PD800_bvals.txt 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvals

mv -f /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs.tmp 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvecs

mv -f /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvals.tmp 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvals

orientLAS 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz

INFO: input image orientation is LAS

INFO: input image determinant is -9.1875

mri_convert -oni 128 -onj 128 -onk 50 -oid -0.999339 -0.0363145 -0.00163044 
-ojd -0.0363101 0.99508 0.0921851 -okd 0.00172524 -0.0921834 0.995741 -oc 
7.38609 19.7949 1.17333 -rt nearest 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz

mri_convert.bin -oni 128 -onj 128 -onk 50 -oid -0.999339 -0.0363145 -0.00163044 
-ojd -0.0363101 0.99508 0.0921851 -okd 0.00172524 -0.0921834 0.995741 -oc 
7.38609 19.7949 1.17333 -rt nearest 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz

normalizing out_i_direction: (-0.999339, -0.0363145, -0.00163044) -> 
(-0.999339, -0.0363145, -0.00163044)

normalizing out_j_direction: (-0.0363101, 0.99508, 0.0921851) -> (-0.0363101, 
0.99508, 0.0921851)

normalizing out_k_direction: (0.00172524, -0.0921834, 0.995741) -> (0.00172524, 
-0.0921834, 0.995741)

$Id: mri_convert.c,v 1.226 2016/02/26 16:15:24 mreuter Exp $

reading from 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz...

TR=0.00, TE=0.00, TI=0.00, flip angle=0.00

i_ras = (-0.999339, -0.0363145, -0.00163044)

j_ras = (-0.0363101, 0.99508, 0.0921851)

k_ras = (0.00172524, -0.0921834, 0.995741)

Reslicing using nearest

writing to 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz...

INFO: found 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvals, 
copying

INFO: found 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvecs, 
converting to LAS

mv -f 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.bvecs
 /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs

mv -f 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.bvals
 /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvals

eddy_correct 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz
 /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.nii.gz 0

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0000

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0001

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0002

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0003

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0004

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0005

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0006

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0007

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0008

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0009

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0010

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0011

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0012

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0013

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0014

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0015

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0016

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0017

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0018

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0019

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0020

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0021

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0022

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0023

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0024

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0025

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0026

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0027

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0028

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0029

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0030

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0031

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0032

processing 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_tmp0033

mv -f /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs.norot

xfmrot 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.ecclog 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs.norot 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs

R: Subscript out of range.

R: Subscript out of range.

R: Subscript out of range.

ln -sf 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/data.nii.gz

mri_convert --frame 34 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/lowb.nii.gz

mri_convert.bin --frame 34 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/lowb.nii.gz

$Id: mri_convert.c,v 1.226 2016/02/26 16:15:24 mreuter Exp $

reading from 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi.nii.gz...

TR=1000.00, TE=0.00, TI=0.00, flip angle=0.00

i_ras = (-0.999339, -0.0363145, -0.00163044)

j_ras = (-0.0363101, 0.99508, 0.0921851)

k_ras = (0.00172524, -0.0921834, 0.995741)

keeping frame 34

   ERROR: valid frame numbers are between 0 and 33

Darwin Christinas-iMac.local 14.5.0 Darwin Kernel Version 14.5.0: Thu Apr 21 
20:40:54 PDT 2016; root:xnu-2782.50.3~1/RELEASE_X86_64 x86_64


trac-preproc exited with ERRORS at Thu Aug 31 14:54:59 CDT 2017


Here is the error I encounter when running the original dicom files through 
tracula


[Christinas-iMac:Freesurfer_temp/PD800/diffusion_recons] christina% trac-all 
-prep -c /Volumes/CFERRIER/Freesurfer_temp/PD800/dmrirc.tutorial

INFO: SUBJECTS_DIR is /Volumes/CFERRIER/Freesurfer_temp/PD800/diffusion_recons

INFO: Diffusion root is /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT

Actual FREESURFER_HOME /Applications/Freesurfer

trac-preproc -c 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/dmrirc.local 
-log 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/trac-all.log 
-cmd 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/scripts/trac-all.cmd

#-------------------------------------

/Applications/Freesurfer/bin/trac-preproc

#-------------------------------------

#@# Image corrections Thu Aug 31 15:31:12 CDT 2017

mri_convert --bvec-voxel 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz

mri_convert.bin --bvec-voxel 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz

$Id: mri_convert.c,v 1.226 2016/02/26 16:15:24 mreuter Exp $

reading from /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm...

Starting DICOMRead2()

dcmfile = /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

dcmdir = /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips --

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

break DICOMRead.c:5228

Ref Series No = 601

Found 5 files, checking for dicoms

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips --

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

break DICOMRead.c:5228

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD806_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips Healthcare--

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD806_DTI.dcm

break DICOMRead.c:5228

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD809_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips Healthcare--

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD809_DTI.dcm

break DICOMRead.c:5228

Found 1 dicom files in series.

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips --

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm

break DICOMRead.c:5228

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD806_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips Healthcare--

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD806_DTI.dcm

break DICOMRead.c:5228

WARNING: tag image orientation not found in 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD809_DTI.dcm

ERROR: don't know how to get DWI parameters from --Philips Healthcare--

ERROR: GetDICOMInfo(): dcmGetDWIParams() 1

DICOM File: /Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD809_DTI.dcm

break DICOMRead.c:5228

First Sorting

Computing Slice Direction



 WARNING: it appears that the image position for all slices is the same.

 This is a problem with the DICOM file. Using direction cosines from col and row

 to compute slice direction, but the output may be misoriented.


Vs: 0 0 0

Vs: nan nan nan

Second Sorting

IsDWI = 0, IsPhilipsDWI = 0

Counting frames

nframes = 1

nslices = 1

ndcmfiles = 1

PE Dir = UNKNOWN (dicom read)

Loading pixel data

TR=0.00, TE=0.00, TI=0.00, flip angle=0.00

i_ras = (-0, -0, 0)

j_ras = (-0, -0, 0)

k_ras = (nan, nan, nan)

writing to 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz...

mri_probedicom --i 
/Volumes/CFERRIER/Freesurfer_temp/PD800/orig_nii/PD800_DTI.dcm > 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dcminfo.dat

cp /Volumes/CFERRIER/Freesurfer_temp/PD800/bvecs/PD800_bvecs.txt 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvecs

cp /Volumes/CFERRIER/Freesurfer_temp/PD800/bvals/PD800_bvals.txt 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvals

mv -f /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvecs.tmp 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvecs

mv -f /Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/bvals.tmp 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.bvals

orientLAS 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig.nii.gz 
/Volumes/CFERRIER/Freesurfer_temp/PD800/TRAC_OUTPUT/PD800/dmri/dwi_orig_las.nii.gz

mat = NULL!

MatrixFree: NULL mat POINTER!


mat = NULL!

MatrixFree: NULL mat POINTER!


mat = NULL!

MatrixFree: NULL mat POINTER!


INFO: input image orientation is III

INFO: input image determinant is nan

order: Subscript out of range.

Darwin Christinas-iMac.local 14.5.0 Darwin Kernel Version 14.5.0: Thu Apr 21 
20:40:54 PDT 2016; root:xnu-2782.50.3~1/RELEASE_X86_64 x86_64


trac-preproc exited with ERRORS at Thu Aug 31 15:32:20 CDT 2017



Thanks for your help. We tried to go through everything but nothing seems to be 
working.
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