Hi everyone again,

I transformed the surfaces from fsaverage to subject's space fine but I got
stuck on the subcortical volume transformation. I apologize, as this is
probably basic, still I run out of ideas...

So, my problem is to transform the 'sig.nii.gz' map (of the subcortical
activations from standard FS-Fast pipeline) from the mni305 to the orig
space of the subject.
I found the 'talairach.xfm' transform from the orig->MNI space, but I
didn't find the reversed registration file (if it exists anywhere, please
let me know). So I created the back-registration file with bbregister:
*bbregister --s Sub001 --mov
~/Freesurfer/subjects/fsaverage/mri/mni305.cor.mgz --T1 --init-fsl --reg
mni2subject.dat*

I checked both with the tkregister2 and with the freeview that the orig.mgz
and the mni305 with applied registration align. So I tried to apply this
registration parameters to the sig.nii.gz with mri_vol2vol:
*mri_vol2vol --mov sig.nii.gz --targ
~/Freesurfer/subjects/Sub001/mri/orig.mgz --reg mni2subject.dat --o
sig.native.nii.gz*
But the output 'sig.native.nii.gz' does not align with the 'orig.mgz' of
the subject.

What's more, the prompt on the terminal suggests to check for the
registration with:
*tkregister2 --mov sig.native.nii.gz --targ
/home/pati/Freesurfer/subjects/Sub001/mri/orig.mgz --reg
sig.native.nii.gz.reg*

as if it was the output file (sig.native...) being moved with the
registration parameters. So I assume that I've messed up something?

So all in end I have a strange situation when sig.nii aligns with MNI, MNI
with registration aligns with orig.mgz, but sig.nii with registration does
not align with orig.mgz.
I will appreciate any ideas of - how to deal with the problem. Also, as it
is highly possible, maybe there's an easier way of transforming the volume
output of fs-fast back to orig subject space?

I'm running Freesurfer 5.3 on Gentoo Linux.

Best regards,
Patrycja

2017-04-26 23:20 GMT+02:00 Bruce Fischl <fis...@nmr.mgh.harvard.edu>:

> glad it worked out :)
>
> On Wed, 26 Apr 2017, Patrycja Naumczyk wrote:
>
> Hi Bruce,
>>
>> thanks for a quick reply! mri_surf2surf worked fine just with specifying
>> the
>> target and source subjects, no registration file needed - much appreciated
>> :)
>>
>> Best regards,
>> Patrycja
>>
>> 2017-04-26 22:51 GMT+02:00 Bruce Fischl <fis...@nmr.mgh.harvard.edu>:
>>       Hi Patrycja
>>
>>       the standard transform we use to fsaverage coordinates is
>>       surface-based (spherical). If you sample your EPI onto the
>>       surface, I believe you can use mri_surf2surf to transfer it from
>>       subject to fsaverage
>>
>>       cheers
>>       Bruce
>>       On Wed, 26 Apr 2017, Patrycja Naumczyk wrote:
>>
>>             Hello FS experts,
>>
>>             I have a question regarding FS-Fast processing. I
>>             have performed
>>             (successfully!)  an FS-Fast single subject analysis.
>>             The results look fine
>>             in the fsaverage space, but I'm interested in
>>             viewing them in the subject
>>             space.
>>
>>             Are the registration files used along the processing
>>             pipeline
>>             (EPI->T1->fsaverage) stored anywhere (so far I only
>>             found the EPI->T1 and
>>             T1->talairach files), so I can use them? Or do I
>>             need to create new
>>             registration files (bbregister/tkregister2)?
>>
>>             Best regards,
>>             Patrycja
>>
>>
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