you can actually skip the ico thing and just use --trgsubject fsaverage6


On 9/28/16 7:06 PM, Jared P Zimmerman wrote:
Thanks Doug,

This does seem like it will do the right thing, but it’s failing because it’s looking for /SUBJECTS_DIR/ico/ and I do not seem to have an ico subject in my subjects_dir

Should this normally be in the subjects_dir? I don’t recall ever seeing this made when I’ve run recon-all in the past.

Thanks,
Jared

Here’s the full terminal output when I run the command:

mri_vol2surf --mov /func_test/filtered_func_residuals.nii.gz --reg func_test/coreg/fs_ep2struct_fsl.dat --hemi lh --o func_test/surface/lh_surf_filtered_func_residuals.mgh --projfrac 0.5 --interp trilinear --noreshape --trgsubject ico --icoorder 6 --out_type mgh --surf-fwhm 6
IcoOrder = 6, nIcoVtxs = 40962
srcvol = /func_test/filtered_func_residuals.nii.gz
srcreg = func_test/coreg/fs_ep2struct_fsl.dat
srcregold = 0
srcwarp unspecified
surf = white
hemi = lh
trgsubject = ico
surfreg = sphere.reg
ProjFrac = 0.5
thickness = thickness
reshape = 0
interp = trilinear
float2int = round
GetProjMax = 0
INFO: float2int code = 0
Done loading volume
Reading surface /subjdir_test//subID/surf/lh.white
Done reading source surface
Reading thickness /subjdir_test//subID/surf/lh.thickness
Done
Mapping Source Volume onto Source Subject Surface
1 0.5 0.5 0.5
using old
Done mapping volume to surface
Number of source voxels hit = 11345
Reading source surface registration
subjdir_test//subID/surf/lh.sphere.reg
Done loading source registration surface
Reading icosahedron, order = 6, radius = 100
  Reading icosahedron /share/apps/freesurfer/5.3.0/lib/bem/ic6.tri
Done loading target registration surface
Mapping Surfaces (subID -> ico)
surf2surf_nnfr: building source hash (res=16).
Surf2Surf: Forward Loop (40962)

surf2surf_nnfr: building target hash (res=16).
Surf2Surf: Reverse Loop (112745)
Reverse Loop had 72236 hits

On Sep 28, 2016, at 3:34 PM, Douglas Greve <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:

You need to specify --trgsubject ico in mri_vol2surf


On 9/28/16 3:01 PM, Jared Zimmerman wrote:
Hi All, forwarding this message along again as I'm still having some trouble getting this to work. Basically I would like to 1) project processed residual time-series from the subject-space volume to the subject-space surface, 2) smooth on the surface, 3) down-sample to FS4 space.

To do this is have used bbregister to calculate the registration, then mri_vol2surf to project to the surface, but I am not sure the output of mri_vol2surf is what I should be expecting. The output file has dimensions 112745 x 1 x 1 x 120 when I'm expecting ~40k vertices for FS6 space and ~3k vertices for FS4 space, but changing the --icoorder flag seems to have no effect on the number of vertices in the output.

I have loaded the output surface functional image into Matlab to se that there is real time-series data in there, but I have no way of knowing if it has mapped the data appropriately. Additionally, I've tried to use mri_surf2surf to downsample this data but have not ben able to successfully get that to downsample the surface. The mri_surf2surf call I used is as follows:

mri_surf2surf --srcsubject subjID --srcsurfval /mov_dir/filtered_func_residuals_fs6_lh_6mm_noreshape.mgh --trgsubject subjID --trgsurfval /mov_dir/filtered_func_residuals_fs4_lh_6mm_noreshape.mgh --hemi lh --trgicoorder 4

Any advice on how to achieve my goal of projecting to subject surfaces, smoothing and downsampling to FS4, as well as recommendations on how to visualize the surface functional data and confirm that the projections has worked properly would be much appreciated. Additionally, what should the dimensions of a surface time series look like? I would have expected for have an nVertices x nFrames x 1 array instead of the nVertices x 1 x 1 x nFrames array, is this correct?


Thank you,
Jared

----- Forwarded Message -----
From: "Jared Zimmerman" <jar...@mail.med.upenn.edu <mailto:jar...@mail.med.upenn.edu>> To: freesurfer@nmr.mgh.harvard.edu <mailto:freesurfer@nmr.mgh.harvard.edu>
Sent: Monday, September 19, 2016 8:46:17 PM
Subject: [Freesurfer] Projecting processed 4D functional data to individualsurfaces

Hi All,

I'm trying to project some processed resting-state data onto the surface to run some surface based parcellations, and I'm running into a bit of difficulty. Basically I'm using bbregister to register my functional to the T1, then mri_vol2surf to project to the surface with the following pseudocode:

bbregister --s subjID --mov /mov_dir/subjID_example_func_brain.nii.gz \
 --reg /mov_dir/coreg/fs_ep2struct_fsl.dat \
 --init-fsl --bold

mri_vol2surf --src /mov_dir/filtered_func_residuals.nii.gz \
 --out /mov_dir/filtered_func_residuals_fs6_lh_6mm_noreshape.mgh \
 --srcreg /coreg/fs_ep2struct_fsl.dat --hemi lh \
--surf white --projfrac 0.5 --icoorder 6 --fwhm 6 --out_type mgh --noreshape


The registration looks pretty good after I run bbregister, but what I get out from the mri_vol2surf seems weird. First of all, the dimensions are 112745 x 1 x 1 x 120, which seems odd. I'm forcing no reshaping, and from the help page it seems like the x-dim should be 40962 for icoorder=6. I have 120 frames in my input data, so that seems to be right, but I'm not sure where this dimension is coming from. I've also tried it with icoorder=4 and I get the same x-dim=112745 when I'm expecting 2562. I've also run these commands with various iterations e.g. reshaping, no smoothing, no projfrac, and I get the same thing every time.

I'm also wondering how I should view these files to confirm that the surface projection has worked as I expect it if/when I am able to get the dimensions to be correct. I cannot open any of the projected functional files in either freeview or tksurfer, so it will be helpful to know how I might be able to view these time-series on the surface after I've succeeded in projecting them to that space.

Any advice would be much appreciated


Thanks,
Jared
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contains patient information, please contact the Partners Compliance HelpLine at
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