Dear FS experts,

I have a question about extracting average cortical thickness from a manually 
created ROI. I am comparing 2 groups, namely PostFort and PreFort and my fsgd 
file appears as the following:

GroupDescriptorFile 1
Title PostPreFortification
Class PostFort
Class PreFort

Input    Subject1        PostFort
Input    Subject2        PostFort
.
.
.

The contrast that I use to compare these groups is [1 -1]. When I display the 
significance map for this contrast I get the following image on the left 
hemisphere:

[X]


My first question is, those blue clusters represent regions, where thickness is 
greater in the PreFort group, don't they? Assuming this is correct, I created a 
label for this region (which contains about 3000 vertices) and extracted the 
thickness value from this region for each subject using the following commands. 
These commands are part of scripts in which $subject and $label are defined.

[X]

to make a label for each subject (the original label was created on fsaverage):
mri_label2label --srclabel $SUBJECTS_DIR/labels/${label}.label --srcsubject 
fsaverage --trglabel $SUBJECTS_DIR/$subject/label/$label.label --trgsubject 
$subject --regmethod surface --hemi lh

to create a stats file of this ROI containing several anatomical measures:
mris_anatomical_stats -f 
$SUBJECTS_DIR/$subject/stats/lh.NP_PostMINUSPre_IPS.label.stats -l 
$SUBJECTS_DIR/$subject/label/NP_PostMINUSPre_IPS.label $subject lh

to compile thickness values from all subjects into one table:
aparcstats2table --hemi lh --subjectsfile 
$SUBJECTS_DIR/Subject_Files/RPDR/214_PrePostFort --parc 
NP_PostMINUSPre_IPS.label --meas thickness --tablefile 
$SUBJECTS_DIR/Analyses_Thickness/Tables/LH_NP_PostMINUSPre_IPS_stats.txt

This table displays the average thickness value of my ROI for each subject. 
Based on this table I calculated the mean thickness for each group, which is as 
the following:

PostFort: 3.043
PreFort: 2.842

My question is: If this cluster appears blue in the Post>Pre map, shouldn't the 
thickness have been greater in the PreFort group? Or am I missing a step?

Many thanks in advance!

Best,
Hamdi

















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