Hi Doug and Bruce,
I posted this question before and I hope that you can help me to find an answer 
for this issue!
I started recently using Tracula to do tractography for my DTI data. I did the 
analysis exactly as mentioned in wiki and every thing ran smoothly. The problem 
started when I was trying to repeat the third step (trac-all -path -c ) for the 
same subjects to generate the statistics; I surprised that Tracula updated all 
the information mentioned in the file "pathstats.overal.txt" regarding the 
tract's volume and the tract's metrics values.
Every time the output is different i.e the statistics regarding the tracts 
volume and the tracts metrics are changing whenever I repeat the third step 
(trac-all -path -c ) of this analysis . This is weird???

Attached are three "pathstats.overal.txt" files for the same subject after 
three consecutive runs for the "trac-all -path -c" (the same subject)


Looking forward for your support
Thanks
Mohamad
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 1151
Len_Min 54
Len_Max 91
Len_Avg 71.1467
Len_Center 70
AD_Avg 0.00143148
AD_Avg_Weight 0.00148653
AD_Avg_Center 0.00146087
RD_Avg 0.000475763
RD_Avg_Weight 0.000414811
RD_Avg_Center 0.000390255
MD_Avg 0.000794336
MD_Avg_Weight 0.000772051
MD_Avg_Center 0.000747128
FA_Avg 0.593211
FA_Avg_Weight 0.653136
FA_Avg_Center 0.664371
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 998
Len_Min 55
Len_Max 105
Len_Avg 70.452
Len_Center 70
AD_Avg 0.00144797
AD_Avg_Weight 0.00148739
AD_Avg_Center 0.00147972
RD_Avg 0.000450739
RD_Avg_Weight 0.000414348
RD_Avg_Center 0.000411816
MD_Avg 0.00078315
MD_Avg_Weight 0.000772027
MD_Avg_Center 0.000767783
FA_Avg 0.614367
FA_Avg_Weight 0.654019
FA_Avg_Center 0.648396
# Title Pathway Statistics
#
# generating_program /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats
# cvs_version 
# cmdline /usr/local/freesurfer/stable5_3_0/bin/dmri_pathstats --intrc 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr
 --dtbase 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dmri/dtifit 
--path fmajor --subj 002201 --out 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.overall.txt
 --outvox 
/autofs/cluster/transcend/malshikho/tractography/tracula_1/002201/dpath/fmajor_PP_avg33_mni_bbr/pathstats.byvoxel.txt
 
# sysname Linux
# hostname icepuff2
# machine x86_64
# user malshikh
# anatomy_type pathway
#
# subjectname 002201
# pathwayname fmajor
#
Count 1500
Volume 693
Len_Min 56
Len_Max 97
Len_Avg 72.1413
Len_Center 29
AD_Avg 0.00150666
AD_Avg_Weight 0.00151482
AD_Avg_Center 0.00130182
RD_Avg 0.000431594
RD_Avg_Weight 0.000428958
RD_Avg_Center 0.000632432
MD_Avg 0.000789951
MD_Avg_Weight 0.000790911
MD_Avg_Center 0.000855561
FA_Avg 0.648118
FA_Avg_Weight 0.653595
FA_Avg_Center 0.457097
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https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer


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