Hi all,

I have been trying to take an SPM volume and extract FS thickness measurements.

I think I successfully took my spm volume from mni152 to fsaverage space 
(mni305) via the command line:

mri_vol2vol --mov $FREESURFER_HOME/subjects/fsaverage/mri.2mm/mni305.cor.mgz 
--targ RTPJ_9mm_001_10_64_-54_16.img --reg 
$FREESURFER_HOME/subjects/fsaverage/mri.2mm/reg.2mm.mni152.dat --inv --o  
$SUBJECTS_DIR/2506/mri/RTPJ_mni305_9mm_001_10_64_-54_16.nii.gz

Then I turned it into an ROI:

mri_binarize --i $SUBJECTS_DIR/2506/mri/RTPJ_mni305_9mm_001_10_64_-54_16.nii.gz 
--o $SUBJECTS_DIR/2506/mri/RTPJ_mni305_roi_9mm_001_10_64_-54_16.nii.gz --min 
.0000000001

> From here I figured I could map it to the fsaverage surface (mri_vol2surf) 
> and extract thickness measurements (mri_segstats using 
> rh.thickness.fsaverage.mgh) as suggested in the volume roi cortical thickness 
> workflow. However, some of my ROIs don't show up after I transform them.  
> From what I can tell, this is because they don't intersect with the fsaverage 
> surface.  

My thinking was that it might be better to stay within the individual subject 
space.  So I would:

1. transform ROI to subject space
2. extract thickness values from rh.thickness  

However, I am struggling to get the roi into single subject space.  I feel like 
this transform (or the inverse of it) should exist because I put all my 
subjects into fsaverage space via the -qcache flag, but i couldn't tell what to 
use.

So I tried:

fslregister --s 2506 --mov $SUBJECTS_DIR/fsaverage/mri/brainmask.mgz --reg 
$SUBJECTS_DIR/2506/fsaverage_to_indiv.dat

But the registration doesn't look all that good.  I am not sure if this is 
because the fsaverage is 'smoothed' or what.

And then I am at a loss for how to put my roi into individual space and then 
extract thickness, or even if this is the right approach.

Thanks,
Stephanie


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