Hi Maryam, I finally figured out what is going on. The bottom line is 
that it is just a weird coincidence. The volume size is 70*70*33=161700 
which happens to be exactly how many vertices are in the left hemisphere 
of this subject. When tksurfer sees this, it thinks that each voxel must 
be a vertex and displays it as such instead of sampling the volume onto 
surface which is why it looks so strange. There is not a way to force 
tksurfer to treat the overlay as a volume, but instead (and probably 
better), you can sample the volume onto the surface, then load it as an 
overlay, eg

mri_vol2surf --reg register.dof6.dat --hemi lh --mov sig.nii.gz --o 
lh.sig.mgh --projfrac 0.5
tksurfer teal_tiger_kcb lh inflated -curv -overlay lh.sig.mgh

and the same thing with the right side.

The chances that this kind of thing happens must be several million to one!
doug



On 09/06/2012 05:26 PM, Maryam Vaziri Pashkam wrote:
> Hi Doug,
>
> I uploaded the tar file. It is called incorrect_lh_test.tar
>
> Thanks,
> Maryam
>
> On Wed, Sep 5, 2012 at 10:04 AM, Douglas N Greve 
> <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>> wrote:
>
>     Can you upload a tarball of the subject and the sig.nii.gz to our
>     file drop?
>
>     www.nmr.mgh.harvard.edu/facility/filedrop/index.html
>     <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>
>     doug
>
>
>
>     On 08/28/2012 10:18 AM, Maryam Vaziri Pashkam wrote:
>
>         Hi Doug,
>
>         Any other insight how I can solve this problem?
>
>         Maryam
>
>         On Fri, Aug 24, 2012 at 7:17 PM, Maryam Vaziri Pashkam
>         <mvaz...@fas.harvard.edu <mailto:mvaz...@fas.harvard.edu>
>         <mailto:mvaz...@fas.harvard.edu
>         <mailto:mvaz...@fas.harvard.edu>>> wrote:
>
>             It looks fine to me. I have attached the tiff image of a
>         coronal
>             slice. So this means that the registration of the surface
>         to the
>             anatomical is fine. This is strange. I tried the tksurfer
>         with the
>             original surface and the overlay on the rh looks fine and
>         the left
>             hemisphere has the same problem as on the inflated
>         surface. Any
>             other idea?
>
>             M
>
>
>             On Fri, Aug 24, 2012 at 6:54 PM, Douglas N Greve
>         <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>> wrote:
>
>
>                 I don't understand your last paragraph. Does the lh
>         surface
>                 look ok, eg, when you run
>
>                 tkmedit teal_tiger_kcb orig.mgz -surfs
>
>                 doug
>
>
>
>
>                 On 08/24/2012 06:37 PM, Maryam Vaziri Pashkam wrote:
>
>                     Yes I have checked it multiple times. They are the
>         same.
>                     Here are the two lines:
>
>                     tksurfer teal_tiger_kcb rh inflated -curv  -overlay
>                    
>         
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/Cat_all/taskvsfix/sig.nii.gz
>                      -overlay-reg
>                    
>         
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/register.dof6.dat
>                     -scalebarflag 1
>
>                     tksurfer teal_tiger_kcb lh inflated -curv  -overlay
>                    
>         
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/Cat_all/taskvsfix/sig.nii.gz
>                      -overlay-reg
>                    
>         
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/register.dof6.dat
>                     -scalebarflag 1
>
>                     I have copy pasted the inflated anatomical folder
>         of the
>                     subject from another computer. Is it possible that
>         some
>                     specific files are not copied properly? I dont
>         know where
>                     are the files that have the information for
>         registration
>                     of the anatomical brain to the inflated suface. My
>         guess
>                     is that is where the problem is. What do you think?
>
>
>                     Maryam
>
>
>
>                     On Fri, Aug 24, 2012 at 6:19 PM, Douglas N Greve
>         <gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>
>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>>> wrote:
>
>
>                         Hi Maryam, it seems very strange that one
>         hemisphere
>                     looks ok but
>                         other looks bad. In fact, I'd say that it cannot
>                     happen. The
>                         differences are not likely to be due to
>         version. Can
>                     you double
>                         check that the tksurfer commands for the lh
>         and rh are
>                     the same
>                         (except for lh and rh, of course)
>                         doug
>
>
>
>
>                         On 08/22/2012 09:13 PM, Maryam Vaziri Pashkam
>         wrote:
>
>                             Hi Doug,
>
>                             I am having a problem with tksurfer overlay
>                     function. I ran
>                             one analysis in freesurfer 5.1 on a mac
>         machine
>                     and I cannot
>                             properly load it on the surface. The
>         analysis was
>                     performed in
>                             the native space. When I use tksurfer to
>         load the
>                     analysis on
>                             the surface using the overlay function I
>         dont get
>                     an error but
>                             the output image just shows small dots on the
>                     surface instead
>                             of patches of activity. It looks like the
>                     registration is not
>                             correct. I checked the registration on the
>         volume
>                     and it looks
>                             fine to me. This only happens with one of my
>                     subject and only
>                             on his left hemisphere. I have attached
>         two tif
>                     images called
>                             lhnative.tif and rhnative.tif that show the
>                     results. I also
>                             ran the analysis on the surface for both
>         left and
>                     right
>                             hemisphere, and it looks fine (lhsurf.tif and
>                     rhsurf.tif files
>                             attached).
>                             Any Idea how to solve this problem? My
>         guess is
>                     that the error
>                             is in the registration of anatomical volume to
>                     anatomical
>                             surface in the left hemisphere of this
>         specific
>                     subject, but I
>                             dont know how to solve this issue.
>
>                             here is the code that I use to load the
>         data on
>                     the surface:
>
>                             tksurfer teal_tiger_kcb lh inflated -curv
>          -overlay
>                                      
>          
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/Cat_all/taskvsfix/sig.nii.gz
>                              -overlay-reg
>                                      
>          
> /Users/maryam/mri-space/studies/SM_PDM_2cat/unpackdata/120421_xu_SM2CAT_06/bold/register.dof6.dat
>                             -scalebarflag 1
>
>                             Just a note that the recon-all was
>         performed in
>                     freesurfer 4.5
>                             and the analysis on freesurfer 5.1. Any chance
>                     this might have
>                             caused the error?
>
>                             Thanks,
>                             Maryam
>
>
>                         --     Douglas N. Greve, Ph.D.
>                         MGH-NMR Center
>         gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>
>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>>
>                         Phone Number: 617-724-2358 <tel:617-724-2358>
>         <tel:617-724-2358 <tel:617-724-2358>>
>         <tel:617-724-2358 <tel:617-724-2358> <tel:617-724-2358
>         <tel:617-724-2358>>>
>                         Fax: 617-726-7422 <tel:617-726-7422>
>         <tel:617-726-7422 <tel:617-726-7422>> <tel:617-726-7422
>         <tel:617-726-7422>
>
>         <tel:617-726-7422 <tel:617-726-7422>>>
>
>                         Bugs:
>         surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
>         <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>         <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>         <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>                         FileDrop:
>         www.nmr.mgh.harvard.edu/facility/filedrop/index.html
>         <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>         <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>         <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>
>
>
>
>                         The information in this e-mail is intended
>         only for
>                     the person to
>                         whom it is
>                         addressed. If you believe this e-mail was sent
>         to you
>                     in error and
>                         the e-mail
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>
>                 --         Douglas N. Greve, Ph.D.
>                 MGH-NMR Center
>         gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>         <mailto:gr...@nmr.mgh.harvard.edu
>         <mailto:gr...@nmr.mgh.harvard.edu>>
>                 Phone Number: 617-724-2358 <tel:617-724-2358>
>         <tel:617-724-2358 <tel:617-724-2358>>
>                 Fax: 617-726-7422 <tel:617-726-7422> <tel:617-726-7422
>         <tel:617-726-7422>>
>
>                 Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
>         <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>         <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>                 FileDrop:
>         www.nmr.mgh.harvard.edu/facility/filedrop/index.html
>         <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>         <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>
>
>
>
>     -- 
>     Douglas N. Greve, Ph.D.
>     MGH-NMR Center
>     gr...@nmr.mgh.harvard.edu <mailto:gr...@nmr.mgh.harvard.edu>
>     Phone Number: 617-724-2358 <tel:617-724-2358>
>     Fax: 617-726-7422 <tel:617-726-7422>
>
>     Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
>     <http://surfer.nmr.mgh.harvard.edu/fswiki/BugReporting>
>     FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html
>     <http://www.nmr.mgh.harvard.edu/facility/filedrop/index.html>
>
>

-- 
Douglas N. Greve, Ph.D.
MGH-NMR Center
gr...@nmr.mgh.harvard.edu
Phone Number: 617-724-2358
Fax: 617-726-7422

Bugs: surfer.nmr.mgh.harvard.edu/fswiki/BugReporting
FileDrop: www.nmr.mgh.harvard.edu/facility/filedrop/index.html

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