Hi,

Many thanks for your reply. Can ypu please advise me how  I should calculate 
the mask if I want to do the masking based upon intensity values found in the 
fa image?

I tried to run (without wmparc) but it gave me error:

mri_mask $TUTORIAL_DIR/$subj/dtrecon/fa.nii \
         $TUTORIAL_DIR/$subj/dtrecon/fa-masked.mgz


Sorry, you should use mri_threshold if you want to do intensity thresholding and mri_mask if you want to use a mask. In the above the second argument, the mask, is missing.


On the tutorial site which is the input volume that it says is not a segmented 
volume during resampling on the CVS:

> mri_vol2vol --targ $FREESURFER_HOME/subjects/cvs_avg35/mri/norm.mgz \
>            --m3z 
$SUBJECTS_DIR/$subj/cvs/combined_tocvs_avg35_elreg_afteraseg-norm.m3z \
>            --noDefM3zPath --reg $TUTORIAL_DIR/$subj/dtrecon/register.dat \
>            --mov $TUTORIAL_DIR/$subj/dtrecon/fa-masked.mgz \
>            --o $TUTORIAL_DIR/$subj/dtrecon/fa-masked.ANAT+CVS-to-avg35.mgz \
>            --interp trilin --no-save-reg

It is "$TUTORIAL_DIR/$subj/dtrecon/fa-masked.mgz"

Based on your experience What will be the best to use when I define my voxels 
the parcellation map or an intensity threshol? 

It really depends on your data. You might want to look at both outputs on a couple of data sets and then decide.

--Lilla


From: Lilla Zollei <lzol...@nmr.mgh.harvard.edu>
To: Antonella Kis <ator...@yahoo.com>
Cc: "freesurfer@nmr.mgh.harvard.edu" <freesurfer@nmr.mgh.harvard.edu>
Sent: Wednesday, January 18, 2012 2:34 PM
Subject: Re: [Freesurfer] How to define the FA-mask


> mri_mask $TUTORIAL_DIR/$subj/dtrecon/fa.nii \
>          $SUBJECTS_DIR/$subj/mri/wmparc2diff.mgz \
>          $TUTORIAL_DIR/$subj/dtrecon/fa-masked.mgz
> and when it follows the resampling on the CVS space I see:
>
> mri_vol2vol --targ $FREESURFER_HOME/subjects/cvs_avg35/mri/norm.mgz \
>            --m3z 
$SUBJECTS_DIR/$subj/cvs/combined_tocvs_avg35_elreg_afteraseg-norm.m3z \
>            --noDefM3zPath --reg $TUTORIAL_DIR/$subj/dtrecon/register.dat \
>            --mov $TUTORIAL_DIR/$subj/dtrecon/fa-masked.mgz \
>            --o $TUTORIAL_DIR/$subj/dtrecon/fa-masked.ANAT+CVS-to-avg35.mgz \
>            --interp trilin --no-save-reg
> with the following explanation: The '--interp' flag indicates the type of 
interpolation we want to use for our resampling. We are not using a segmented 
volume as an input so we use the default trilinear (trilin) interpolation option. 
If you wanted to resample a
> segmentation volume (such as wmparc or aseg) in CVS space, you would want to 
use the nearest neighbor (nearest) interpolation option.
>
> I don't understand what exactly means: "We are not using a segmented volume as an 
input" since the fa-masked is defined using the wmparc2diff?

When you masked the FA volume, you eliminated some values that are outside of 
the mask but did not create a binary volume, the original FA values remained in 
the volume.

> I understood there two  masking options: (option 1) using the  wmparc to 
guide my masking  or (option 2) if I want to do the masking based upon intensity 
values found in the fa image.
> I am not sure how will be the best to define my FA-mask if I was running the 
CVS registration with the option --noaseg and also I want to do a group study and 
see if my Fa values increases or decreases in controls vs. patients. Can you 
please give me an advise?

These two things are not related. When considering the masking the only thing 
you need to think about is whethe ryou trust the parcellation map or an 
intensity threshold better in order to define voxels that you would like to 
keep in your input volume.

>  Also, I am not sure if I am mixing things or not but can I define my mask 
using the wmparc if I did the registration with the --noaseg option?

Again, these deceisions are not related.

Lilla


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