Hello Doug, I transformed the aseg_m file to be 3x3x3. I will try and trouble shot the possible error in that transform-
The AFNI steps I used to transform the freesurfer files to AFNI format, and than to be 3x3x3 are pasted below. Liat ----- foreach subj (xxx) #step1 @SUMA_Make_Spec_FS -sid ${subj} #step2 @SUMA_AlignToExperiment \ -exp_anat /Volumes/cbgb/Liat/freesurfer_subjects/${subj}/SUMA/${subj}_anat+orig \ -surf_anat /Volumes/cbgb/Liat/freesurfer_subjects/${subj}/SUMA/${subj}_SurfVol+orig \ -wd \ -prefix ${subj}_AlignExp #step 3: To align a given parcellation file 3dWarp -NN -matparent ${subj}_AlignExp+orig -prefix ${subj}_aseg_m aseg.nii #step 4: I transformed the functional data set by the talairch transform obtained for the anatomical data, and then which was resampled to a 3x3x3 space during this step. @auto_tlrc -apar ${subj}_anat_at+tlrc -input ${subj}_FC_Seed_aseg_fs_AMY_stats_ni_pi_Final+orig -dxyz 3 -suffix _at3 end ----- Original Message ----- From: Doug Greve <[EMAIL PROTECTED]> Date: Monday, July 28, 2008 1:07 pm Subject: Re: [Freesurfer] AFNI /freesurfer aseg files problem > > I would suspect that something happened when you converted to afni > format. The aseg starts out at 1mm3, 256^3, but it appears to be > 3mm3, > 54x64x50. These conversions are quit tricky and easy to get wrong. > Also, > did you check the aseg.stats file to assure that the volumes of the > amyg > are more-or-less symetrical? > > doug > > > R-to-L extent: -79.500 [R] -to- 79.500 [L] -step- 3.000 mm > [ 54 > voxels] > A-to-P extent: -79.500 [A] -to- 109.500 [P] -step- 3.000 mm > [ 64 > voxels] > I-to-S extent: -63.500 [I] -to- 83.500 [S] -step- 3.000 mm > [ 50 > voxels] > > > Liat Levita wrote: > > >Hi Doug, I just checked, yes they are. > > > >Liat > > > >PS. I attached the Info files to this message. > > > > > > > > > >----- Original Message ----- > >From: Doug Greve <[EMAIL PROTECTED]> > >Date: Monday, July 28, 2008 12:16 pm > >Subject: Re: [Freesurfer] AFNI /freesurfer aseg files problem > > > > > > > >>are you sure that t1rc and pscA are the same dimension? Remember, > >>freesurfer resamples everything into 1mm3, 256^3 > >> > >>doug > >> > >> > >> > >>Liat Levita wrote: > >> > >> > >> > >>>Hello, > >>>I wonder if anyone using AFNI converted freesurfer files has > come > >>> > >>> > >>across this problem (or can tell me if I making an error > somewhere > >>that I am just not seeing right now). I posted the same question > on > >>the AFNI board but haven't gotten feedback as yet. > >> > >> > >>>Here is the problem: > >>> > >>>After using freesurfer to get subcortical segmentations, I > >>> > >>> > >>converted the resulting freesurfer files to AFNI format, which > was > >>fine - I had no problems doing that. However, when I try use > these > >>subcortical segmentation data files (subjectID_aseg_m_at3+tlrc) > as > >>masks to pull put PSC signal from each region of interest, I am > >>getting an odd effect of right versus left; whereby left clusters > >>output is fine ( voxel numbers are correct after 3dmaskave), but > >>for the left it is very wrong. I have checked that the labels of > >>the ROIs I am using are correct on AFNI GUI and they are. (code > and > >>output below) > >> > >> > >>>I am a bit at a loss; any help will be much appreciated. > >>> > >>>Liat > >>> > >>>AFNI Code used > >>>--------------- > >>>Foreach subj (xxx) > >>>foreach cluster (18 54) > >>>3dmaskave -q -mask ${subj}_aseg_m_at3+tlrc -mrange ${cluster} > >>> > >>> > >>${cluster} \ > >> > >> > >>>"${subj}_all_runs_pscA_at3+tlrc" > > >>> > >>> > >>${subj}_fsROI_PSC_cluster_${cluster}.1D > >> > >> > >>>end > >>>end > >>> > >>>Output (get the same odd voxel output from all right hand side > of > >>> > >>> > >>the brain freesurfer generated ROIs) > >> > >> > >>>------------------- > >>>*For cluster 18 Left Amygdala > >>>++ 3dmaskave: AFNI version=AFNI_2008_02_01_1144 (Jul 3 2008) [32- > bit]>>>+++ 37 voxels survive the mask > >>> > >>>*For cluster 54 Right Amygdala > >>>++ 3dmaskave: AFNI version=AFNI_2008_02_01_1144 (Jul 3 2008) [32- > bit]>>>+++ 200 voxels survive the mask > >>> > >>> > >>> > >>> > >>>_______________________________________________ > >>>Freesurfer mailing list > >>>Freesurfer@nmr.mgh.harvard.edu > >>>https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer > >>> > >>> > >>> > >>> > >>> > >>> > >>-- > >>Douglas N. Greve, Ph.D. > >>MGH-NMR Center > >>[EMAIL PROTECTED] > >>Phone Number: 617-724-2358 > >>Fax: 617-726-7422 > >> > >>In order to help us help you, please follow the steps in: > >>surfer.nmr.mgh.harvard.edu/fswiki/BugReporting > >> > >> > >>_______________________________________________ > >>Freesurfer mailing list > >>Freesurfer@nmr.mgh.harvard.edu > >>https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer > >> > >> > >> > > -- > Douglas N. Greve, Ph.D. > MGH-NMR Center > [EMAIL PROTECTED] > Phone Number: 617-724-2358 > Fax: 617-726-7422 > > In order to help us help you, please follow the steps in: > surfer.nmr.mgh.harvard.edu/fswiki/BugReporting > > > _______________________________________________ Freesurfer mailing list Freesurfer@nmr.mgh.harvard.edu https://mail.nmr.mgh.harvard.edu/mailman/listinfo/freesurfer