Hi Eric,

Message: 1
Date: Wed, 10 May 2006 09:37:43 -0400
From: "Faden, Eric (NIH/NIMH) [F]" <[EMAIL PROTECTED]>
Subject: [Freesurfer] Skull Stripping a Macaque
To: <freesurfer@nmr.mgh.harvard.edu>
Message-ID:
        <[EMAIL PROTECTED]>
Content-Type: text/plain;       charset="iso-8859-1"

Hey all,

Is anyone using freesurfers mri_watershed to strip macaque skulls?
Yes, some people are, there was even a short tutorial/howto posted on this very list (maybe you can find it in the archives).

If so what options are you using?
Inlined after your mail you can find the script I use for skull stripping. The take home message from the howto, at least for me, was to try different sets of parameters with "-surf_debug" to nicely visualize the results. Unfortunately I got the best results so far only with an additional step of manual editing of the brainmask, as my scans suffer from inhomogenities (surface coil used for transmit and receive). For the manual edit steps I recommend the fine skull stripping howto found somewhere at the freesurfer wiki. One thing that caused slight hickups for me, was rĀ“the fact that the erase funtion of the volume brush does not write zeros but ones, which messed up the segmentation later on, as the voxels with a value of one got much brighter by the normalisation step, but I digress...

I am currently trying to use it but am getting really bad results.
        Care to compare brainmasks to see what you consider bad?

I am also open to suggestions of other programs to try.

-Eric

Ahoi
        Sebastian

------Cut and paste from here-----
#! /bin/csh -f # use tkmedit to mark critical whitematter points #setenv FIX_VERTEX_AREA # set the source directory... set src_dir = ../mri # set the target directory and file_name set targ_dir = ../mri #set reg = rotate_conf_2_atlas.dat #set targ_vol = rawavg.mgz #set controlpoints = ../tmp/control.dat set in_vol = ${src_dir}/nu.mgz #orig.mgz set t1 = ${targ_dir}/T1.mgz # do the skull stripping # repeat with -surf_debug until the parameters are okay, the run without... #mri_watershed -surf_debug -atlas ${targ_dir}/${t1} ${targ_dir}/brainmask.auto.mgz #mri_watershed -surf_debug -atlas -wat+temp ${targ_dir}/${t1} ${targ_dir}/brainmask.auto.mgz #mri_watershed -surf_debug -h 25 -wat+temp ${targ_dir}/${t1} ${targ_dir}/brainmask.auto.mgz #mri_watershed -h 10 -wat -n ${targ_dir}/${t1} ${targ_dir}/brainmask.auto.mgz mri_watershed -atlas ${targ_dir}/${t1} ${targ_dir}/brainmask.auto.mgz # create the brainmask ##cp ${targ_dir}/brainmask.auto.mgz ${targ_dir}/brainmask.mgz # check results... tkmedit -f ${targ_dir}/brainmask.mgz -aux ${targ_dir}/T1.mgz #cp ${t1} ${targ_dir}/brain.mgz # does a register file already exist? #if !(-e ${targ_dir}/${reg}) then # cp ./${reg} ${targ_dir}/${reg} #endif # convert to cor? or turn this sucker so that it works with mgz? #TODO: normalize with control points...
----Cut and paste until here---------------

--
Sebastian Moeller

Tel.: 04 21 - 2 18 - 78 38 oder 96 91
Fax.: 04 21 - 2 18 - 90 04
GSM:  01 62 - 3 25 45 59
[EMAIL PROTECTED]

AG Kreiter / FB 2
Institut fuer Hirnforschung III
Abteilung Theoretische Neurobiologie
Universitaet Bremen
Biogarten
Hochschulring 16a
Postfach 33 04 40
28359 Bremen



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