The first org mode file opened (.txt suffix) is not colorized. To force colorize I use 'org-mode-restart. Subsequently opened txt files are properly colorized. This happens under Windows 10 using Emacs 27.1 and 27.2, and org-mode 9.3.7 and 9.4.5. There are no problems under Ubuntu Linux.
Oddly, the problem does not appear on my new desktop (Lenovo). It happens on my ASUS laptop. I'm using the same version of org-mode and Emacs on both machines. I copied .emacs.d from the desktop to the laptop to be sure the environments are the same, and I installed Emacs 27.1 and 27.2 on the laptop. The colorize problem occurs on the laptop no matter the environment. It also occurred with my old desktop.