Dear all, there are only 4 seats left for the upcoming Physalia online course on "Assembly and Annotation of Genomes". Dates: Online, March 17th-21st Course website: [ https://www.physalia-courses.org/courses-workshops/course20/ ]( https://www.physalia-courses.org/courses-workshops/course20/ ) This course will introduce biologists and bioinformaticians to the concepts of de novo genome assembly and annotation, providing a theoretical framework and practical examples. A variety of sequencing technologies and their applications to generate haplotype-phased, high-quality reference genomes will be presented and discussed. They include Illumina short reads (for both assembly and gene annotation), PacBio HiFi (‘High Fidelity’) and CLR (‘Continuous Long Read’) reads, Oxford Nanopore long and ultralong reads, as well as scaffolding technologies including optical mapping and proximity ligation (Hi-C). Special attention will be given to quality control throughout the assembly process (e.g. tools such as Genomescope, Merqury, Pretext) as well as to consensus, structural error mitigation and manual curation. The concept of Telomere-to-telomere (T2T) genome assembly, and the means to achieve it, will also be introduced. Annotation tools using Illumina RNA-Seq and Pacbio IsoSeq data will be introduced. By the end of the course the students will be able to understand what is needed to generate an annotated and curated reference genome of high-quality. For the full list of our courses and workshops, please visit: [ https://www.physalia-courses.org/courses-workshops/ ]( https://www.physalia-courses.org/courses-workshops/course20/ ) Best regards, Carlo --------------------
Carlo Pecoraro, Ph.D Physalia-courses DIRECTOR i...@physalia-courses.org To unsubscribe from this list please go to https://community.esa.org/confirm/?u=RhPWqPxFwODKvbkiT32nkIqRrsiSgulp
