Am 25.03.21 um 22:00 schrieb Nilesh Patra: > On 2021-03-26 01:58, Andreas Tille wrote: >> Hi folks, >> >> I have packaged bamclipper[1] since it is a precondition for the >> pipeline covpipe[2] which is developed and used in my institute. >> (@Steffen, possibly another column in the spreadsheet.) Please add it. >> Despite bamclipper is pretty easy I would love to have some autopkgtest >> but I'm lacking the needed files (bam + bam.bai as well as bedpe). The >> README.md[3] has an example that could be used as autopkgtest - so it >> should not be a hard job for someone who has such files / knows how to >> find some examples. > I found those files here[1] inside examples/ dir -- it also has > outputted *.primer.bam* files, so I think it is sensible > to assume that these files work. > I follow this in other packages as well, when on adding certain bam or > sam files, "something" happens which is good for > at least a preliminary functioning test :-) > > I added a autopkgtest to the salsa repo, please take in a look. > > PS: Please consider enabling salsa CI for any new packages that you > might push. I did so for this one for now. > > [1]: https://github.com/tommyau/bamclipper
https://software.broadinstitute.org/software/igv/BAM One of the many reads from a high-throughput sequencing machine (pre-Nanopore this was 30, 70 or 144 nucleotides in length, since the nanopore we are talking about 10^6 "basepairs" that are continuously sequenced) is just a series of characters. For every such read you want to know where in the reference genome it likely was sequenced from, how the quality was at different parts of the read and how well it matches against a reference genome. bowtie http://bowtie-bio.sourceforge.net/manual.shtml gets reads as input, writes SAM which are converted to BAM via "samtools view". There is loads of public data out there that we could use for testing purposes. As long as we can automate the generation of such a Debian Med Testing Environment from public data - there is no need to have everything that we test on as a Debian package, right? Best, Steffen
