You are nit accidentally looking at the anomalous difference map are you ?
On Fri, 5 Sep 2025 at 10:39, Catherine Back <
[email protected]> wrote:
> Good morning,
>
> I am currently solving a structure containing four heme b molecules (res
> 1.7 Å), each coordinated by two histidines. The refinement is looking good,
> but the output from refinement has marked the Fe ions of each heme with
> positive density (green) in the FoFc difference map - see image. Any ideas
> why? I used the Monomer Library in Coot to add hemes ('HEM') in. Is it
> something to do with the oxidation state of the Fe? And if so, is there
> anything I can do about it?
>
> Best wishes,
> Catherine
>
> Dr Catherine R. Back (she/her)
>
> Senior Post-doctoral Research Associate
>
> School of Biochemistry
> University of Bristol
>
> UK
>
>
>
>
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