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MARK WILSON Department of Biochemistry University of Nebraska WEDNESDAY, April 23, 13:30 (EST) Electrostatically Gated Enzyme Dynamics During Catalysis Register in advance for this meeting: https://bnl.zoomgov.com/meeting/register/meyb6iiXRqWCXEW03dHvbg Time conversion Link: https://www.worldtimebuddy.com/<https://urldefense.com/v3/__https:/gcc02.safelinks.protection.outlook.com/?url=https*3A*2F*2Fwww.worldtimebuddy.com*2F&data=05*7C01*7Cgenoa.blankenship*40pnnl.gov*7C67ffe754733240e42d3e08da641ba4bc*7Cd6faa5f90ae240338c0130048a38deeb*7C0*7C0*7C637932367171794987*7CUnknown*7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0*3D*7C3000*7C*7C*7C&sdata=aSe2IUSomjge9ZW6zXdQ81fPzxpg8eawtRkoiOEJdXM*3D&reserved=0__;JSUlJSUlJSUlJSUlJSUlJSUlJSU!!P4SdNyxKAPE!Fd5kIAbibocFpkUaEbpqZZGm8tw-xiqb5oC4sfOklv6Kbbf-T3RvUobMSLrzyXNbfNVT9vMxJGuY1RsSqbc6MjpIABre1Q$> Abstract: Enzyme catalysis is essential for life and is a central phenomenon in biochemistry. The advent of time-resolved serial crystallography, initially enabled by X-ray free electron lasers (XFELs) and now expanding to synchrotron X-ray sources, allows enzyme catalysis to be observed in real time, in near-physiological conditions, and at atomic resolution. I will describe our work using mix-and-inject serial crystallography (MISC) to observe catalysis by isocyanide hydratase (ICH). MISC allowed us to observe formation of an unusual thioimidate intermediate and to watch ICH’s conformational dynamics respond to changes in active site ionization during catalysis. We also used an engineered ICH mutant to enrich for rare conformations during catalysis, permitting a clearer view of later steps in the reaction. ICH exemplifies a class of enzymes whose non-equilibrium dynamics are gated by changes in active site electrostatics, which is a potentially common enzymological phenomenon. ================= Vivian Stojanoff, PhD Education, Training, Outreach User Program p 1(631) 344 8375 e lifescie...@bnl.gov<mailto:lifescie...@bnl.gov> w https://www.bnl.gov/ps/lifesciences/<https://www.bnl.gov/ps/lsbr/> Address: Center for Biomolecular Structure National Synchrotron Light Source II Building 745 Brookhaven National Laboratory Upton NY 11973 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
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