What’s the net charge of your peptide? By any chance positive? Then you have non specific binding to the chip through electrostatic interaction. Jürgen __________________________________________ Jürgen Bosch, PhD, MBA Center for Global Health & Diseases Case Western Reserve University
https://www.linkedin.com/in/jubosch/ CEO & Co-Founder at InterRayBio, LLC On Mar 29, 2025, at 11:18, Gopinath Krishnappa <gopii...@gmail.com> wrote: Dear Sir, I have a query regarding the Biacore 1k+ - NTA chip experiment. I have performed an interaction study between a his-tagged protein and peptide. I am facing some problems in the experiment, and I am guessing it is with the EDTA regeneration. The response of the 0 µM concentration (1X PBS) was high compared with all other concentrations of the peptide (125, 62.5, 31.25, 15.6, 7.8, 3.9 µM). I have attached the sensogram for the 125 µM concentration compared with the 0 µM concentration for your reference. Kindly help me with this query. Thanks and regards -- Gopinath K ICMR-SRF Department of Biophysics National Institute of Mental Health and Neurosciences Bengaluru-560029 Ph.No:+919741047738 Email: gopii...@gmail.com ------------------------------ To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 <NTA_overlay.PNG> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
