Thanks to everyone for the quick replies! I’ll start by fitting PEG and let you know how it goes.
Best wishes, Chris On Mon, 3 Feb 2025 at 18:22 Boaz Shaanan <bshaa...@bgu.ac.il> wrote: > So it does to me too. There are a few PEG variants in the monomer library, > have you tried them? Most PEGs, if not all, are mixtures in terms of chain > length. > > Boaz Shaanan, Ph.D. > Dept. of Life Sciences > Ben Gurion University > Beer Sheva, Israel > > On Feb 3, 2025 19:12, Dale Tronrud <de...@daletronrud.com> wrote: > Looks like a pretty good PEG fragment to me. > > Dale Tronrud > > On 2/3/2025 8:42 AM, Chris Fage wrote: > > Dear All, > > > > Our ~2.3 Å crystal structure contains a tube of electron density for an > > unknown ligand, into which we've built a network of water molecules (see > > attached images with 2Fo-Fc map contoured at 1.5 sigma). We'd be > > grateful for any help in identifying that ligand. The > > purification buffer contains HEPES and NaCl, the crystallization > > solution consists of PEG 4000 and MES, and glycerol was used as the > > cryoprotectant. None of those fit the density. Aside from the Ser side > > chain and backbone O atom, which are within H-bonding distance of the > > individual water molecule, most of the nearby protein atoms are C or H. > > Could the density represent an ordered fragment of a PEG chain? Any > ideas?! > > > > Thanks, and best wishes, > > Chris > > > > waters_1.5sigma_a.pngwaters_1.5sigma_b.png > > > > ------------------------------------------------------------------------ > > > > To unsubscribe from the CCP4BB list, click the following link: > > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > <https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1> > > > > ######################################################################## > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > > This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a > mailing list hosted by www.jiscmail.ac.uk, terms & conditions are > available at https://www.jiscmail.ac.uk/policyandsecurity/ > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
