So it does to me too. There are a few PEG variants in the monomer library, have
you tried them? Most PEGs, if not all, are mixtures in terms of chain length.
Boaz Shaanan, Ph.D.
Dept. of Life Sciences
Ben Gurion University
Beer Sheva, Israel
On Feb 3, 2025 19:12, Dale Tronrud <de...@daletronrud.com> wrote:
Looks like a pretty good PEG fragment to me.
Dale Tronrud
On 2/3/2025 8:42 AM, Chris Fage wrote:
> Dear All,
>
> Our ~2.3 Å crystal structure contains a tube of electron density for an
> unknown ligand, into which we've built a network of water molecules (see
> attached images with 2Fo-Fc map contoured at 1.5 sigma). We'd be
> grateful for any help in identifying that ligand. The
> purification buffer contains HEPES and NaCl, the crystallization
> solution consists of PEG 4000 and MES, and glycerol was used as the
> cryoprotectant. None of those fit the density. Aside from the Ser side
> chain and backbone O atom, which are within H-bonding distance of the
> individual water molecule, most of the nearby protein atoms are C or H.
> Could the density represent an ordered fragment of a PEG chain? Any ideas?!
>
> Thanks, and best wishes,
> Chris
>
> waters_1.5sigma_a.pngwaters_1.5sigma_b.png
>
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